{"id":109935,"date":"2026-07-22T15:40:26","date_gmt":"2026-07-22T13:40:26","guid":{"rendered":"https:\/\/rd-biotech.tetedecom.pro\/?page_id=109935"},"modified":"2026-07-27T15:36:49","modified_gmt":"2026-07-27T13:36:49","slug":"recombinant-antibody-production","status":"publish","type":"page","link":"https:\/\/rd-biotech.tetedecom.pro\/en\/antibody-production-service\/recombinant-antibody-production\/","title":{"rendered":"Recombinant antibody production"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;40&#8243; back_image=&#8221;109744&#8243; back_repeat=&#8221;no-repeat&#8221; back_position=&#8221;center center&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;644461&#8243; row_height_pixel=&#8221;400&#8243; overlay_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;1&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;484880&#8243;][uncode_breadcrumbs][vc_empty_space empty_h=&#8221;2&#8243;][vc_custom_heading auto_text=&#8221;yes&#8221; text_size=&#8221;fontsize-155944&#8243; text_weight=&#8221;200&#8243; text_space=&#8221;fontspace-781688&#8243; uncode_shortcode_id=&#8221;497824&#8243;][\/vc_custom_heading][vc_empty_space empty_h=&#8221;2&#8243;][vc_custom_heading text_size=&#8221;h5&#8243; text_weight=&#8221;200&#8243; css_animation=&#8221;alpha-anim&#8221; animation_speed=&#8221;2000&#8243; uncode_shortcode_id=&#8221;843607&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"264\" data-end=\"378\">At RD-Biotech, we specialize in producing high-quality recombinant antibodies with extremely low endotoxin levels.<\/p>\n<p data-start=\"380\" data-end=\"680\">Using our optimized CHO- and HEK-cell expression platforms, we can produce quantities ranging from a few milligrams to several grams across a broad range of antibody formats, based on your sequences of interest. With our Fast-Track production service, delivery is possible in as little as four weeks.<\/p>\n<p>[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;266515&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;the-benefits-of-recombinant-antibodies&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;906540&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;avantages-systeme-hybridomes&#8221;]The benefits of recombinant antibodies[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;40&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;156057&#8243; row_height_pixel=&#8221;400&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;102621&#8243;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;88178&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;5\/12&#8243; uncode_shortcode_id=&#8221;139016&#8243;][\/vc_column_inner][vc_column_inner width=&#8221;7\/12&#8243;][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;132088&#8243; text_color_type=&#8221;uncode-palette&#8221;]<strong>Produced from defined genetic sequences, recombinant antibodies<\/strong> ensure reproducibility and specificity while allowing genetic modifications tailored to advanced scientific and therapeutic applications.[\/vc_column_text][vc_column_text uncode_shortcode_id=&#8221;786331&#8243;]<\/p>\n<p data-start=\"927\" data-end=\"1117\"><strong>Recombinant antibodies<\/strong> offer significant benefits. Unlike <a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/antibody-production-service\/production-of-hybridoma-derived-antibodies\/\">hybridoma-derived antibodies<\/a>, they are produced through an animal-free process in a chemically and biologically defined environment.<\/p>\n<p data-start=\"1119\" data-end=\"1439\">Because they are produced from a <strong>defined genetic sequence<\/strong>, they provide optimal batch-to-batch reproducibility and ensure the long-term availability of scientific reagents. Selection and molecular engineering can generate antibodies with high affinity and specificity for their target, thereby reducing cross-reactivity.<\/p>\n<p data-start=\"1441\" data-end=\"1547\"><strong>Recombinant antibodies<\/strong> can also be easily modified through genetic engineering. This makes it possible to:<\/p>\n<ul data-start=\"1549\" data-end=\"1683\">\n<li data-section-id=\"bu9khk\" data-start=\"1549\" data-end=\"1578\"><strong>Change the antibody isotype<\/strong><\/li>\n<li data-section-id=\"lhuzeo\" data-start=\"1579\" data-end=\"1599\">Add tags or labels<\/li>\n<li data-section-id=\"1dlv4u\" data-start=\"1600\" data-end=\"1632\">Improve affinity and stability<\/li>\n<li data-section-id=\"19eutnn\" data-start=\"1633\" data-end=\"1683\"><strong>Humanize antibodies for therapeutic applications<\/strong><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;105305&#8243; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;recombinant-antibody-formats&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;123902&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;avantages-systeme-hybridomes&#8221;]A wide range of recombinant antibody formats[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;161319&#8243; text_color_type=&#8221;uncode-palette&#8221;]Discover our <strong>diverse antibody formats<\/strong>, including <strong>chimeric<\/strong>, <strong>bispecific<\/strong>, <strong>afucosylated<\/strong> and <strong>humanized recombinant antibodies<\/strong>, antibody fragments and protein fusions.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;140868&#8243; row_height_pixel=&#8221;400&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;185381&#8243;][vc_tabs tab_scrolling=&#8221;yes&#8221; uncode_shortcode_id=&#8221;605913&#8243; el_class=&#8221;tab-services mobile-tabs-to-dropdown&#8221;][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Chimeric antibodies&#8221; tab_id=&#8221;1757666499-1-617775349915352931784795795632&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;0&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;955701&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;0&#8243; border_color=&#8221;transparent&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;472868&#8243; el_class=&#8221;service-box&#8221; border_color_type=&#8221;uncode-palette&#8221; css=&#8221;.vc_custom_1777536877602{border-top-width: 0px !important;}&#8221;][vc_column_text uncode_shortcode_id=&#8221;462647&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" style=\"text-align: left;\" data-start=\"2098\" data-end=\"2274\"><strong>Antibody chimerization<\/strong> involves combining the variable regions of a \u201cparental\u201d or \u201cdonor\u201d antibody with the constant regions of an \u201cacceptor\u201d antibody from a different species.<\/p>\n<p style=\"text-align: left;\" data-start=\"2276\" data-end=\"2417\">The most common combination consists of murine variable domains and human constant domains, although other combinations can also be produced.<\/p>\n<p style=\"text-align: left;\" data-start=\"2419\" data-end=\"2574\"><strong>Chimerization<\/strong> is a simple, effective approach that retains the affinity and specificity of the <strong>parental antibody while reducing the risk of immunogenicity.<\/strong><\/p>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;110987&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;151353&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Bispecific antibodies&#8221; tab_id=&#8221;1772808992395-2-317775349915352921784795795632&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;256557&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;214884&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;200970&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" style=\"text-align: left;\" data-start=\"2602\" data-end=\"2757\">Our expertise also includes the <strong>production of bispecific antibodies<\/strong> capable of simultaneously recognizing and binding to two distinct antigens or epitopes.<\/p>\n<p style=\"text-align: left;\" data-start=\"2759\" data-end=\"2918\">These sophisticated molecules are particularly valuable therapeutic tools, enabling innovative, more precise targeting strategies for the treatment of disease.<\/p>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;110988&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;xl&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;206921&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Afucosylated antibodies&#8221; tab_id=&#8221;1757666499-2-8617775349915356871784795795632&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;256557&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;149752&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;560638&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" style=\"text-align: left;\" data-start=\"2948\" data-end=\"3078\">We provide an <strong>afucosylated antibody production service<\/strong> for <strong>antibodies with enhanced antibody-dependent cellular cytotoxicity<\/strong> &#8211; <strong>ADCC<\/strong>.<\/p>\n<p style=\"text-align: left;\" data-start=\"3080\" data-end=\"3312\">Because these antibodies lack fucose residues in their Fc region, they exhibit improved cellular cytotoxicity. This optimization promotes a <strong>stronger immune response<\/strong>, improves therapeutic efficacy and may help reduce adverse effects.<\/p>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;110989&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;xl&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;190569&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Antibody fragments and VHHs&#8221; tab_id=&#8221;1777535231154-3-64051784795795632&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;256557&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;334868&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;463570&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" style=\"text-align: left;\" data-start=\"3346\" data-end=\"3501\"><strong>Antibody fragments<\/strong> such as <strong>scFv, Fab and F(ab\u2019)<\/strong>\u2082, together with VHHs, offer numerous strategic, scientific and industrial benefits due to their small size.<\/p>\n<p style=\"text-align: left;\" data-start=\"3503\" data-end=\"3695\">Their ability to access otherwise inaccessible epitopes, penetrate tissues more effectively and eliminate Fc-mediated immune effector functions makes these molecules extremely versatile tools.<\/p>\n<ul data-start=\"3697\" data-end=\"3912\">\n<li style=\"text-align: left;\" data-section-id=\"19s9qlf\" data-start=\"3697\" data-end=\"3781\"><strong data-start=\"3699\" data-end=\"3727\">Multi-isotype expertise:<\/strong> purification of IgG, IgM, IgA, IgY and other isotypes<\/li>\n<li style=\"text-align: left;\" data-section-id=\"1schg3d\" data-start=\"3782\" data-end=\"3912\"><strong data-start=\"3784\" data-end=\"3810\">Ready-to-use delivery:<\/strong> antibodies are formulated in your chosen buffer and supplied with a comprehensive purification report<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;110989&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;xl&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;998725&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Humanized antibodies&#8221; tab_id=&#8221;1777882602171-4-101784795795632&#8243;][vc_row_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;691039&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;203712&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"3939\" data-end=\"4032\"><strong>Humanized antibodies<\/strong> now account for the majority of <strong>therapeutic antibodies approved<\/strong> for use.<\/p>\n<p data-start=\"4034\" data-end=\"4199\">A parental antibody is humanized using a modernized version of the <strong>CDR-grafting method developed<\/strong> by Winter <em data-start=\"4141\" data-end=\"4149\">et al.<\/em> at the<a href=\"https:\/\/en.wikipedia.org\/wiki\/Medical_Research_Council_(United_Kingdom)\"> Medical Research Council in Cambridg<\/a>e, UK.<\/p>\n<p data-start=\"4201\" data-end=\"4438\">This approach involves inserting the complementarity-determining regions (<strong>CDRs<\/strong>)of a parental antibody, usually murine, into a <strong>human antibody framework<\/strong>. It preserves antigen specificity and affinity while reducing immunogenicity in humans.<\/p>\n<p data-start=\"4440\" data-end=\"4582\">Following humanization, sequence optimization can be offered, with optional supplementary <strong>molecular-modelling analyses.<\/strong> The objectives are to:<\/p>\n<ul data-start=\"4584\" data-end=\"4718\">\n<li data-section-id=\"rn0wea\" data-start=\"4584\" data-end=\"4637\">Improve manufacturability by reducing heterogeneity<\/li>\n<li data-section-id=\"1qfwmq7\" data-start=\"4638\" data-end=\"4718\">Increase antibody affinity and biological activity, including IC\u2085\u2080 performance<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;110990&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;xl&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;205403&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Recombinant proteins and antibody fusion proteins&#8221; tab_id=&#8221;1777883770378-5-41784795795632&#8243;][vc_row_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;255540&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;165116&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"4768\" data-end=\"4864\">RD-Biotech also applies its expertise to the development and <a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/recombinant-protein-expression\/\">production of recombinant proteins<\/a>.<\/p>\n<p data-start=\"4866\" data-end=\"4995\">These proteins can be fused to an Fc domain to optimize their performance according to the specific requirements of your project.<\/p>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_tab][\/vc_tabs][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;157461&#8243; back_color_type=&#8221;uncode-solid&#8221; back_color_solid=&#8221;#f0fbff&#8221; el_id=&#8221;recombinant-antibody-production-process&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;129206&#8243;][vc_column_inner width=&#8221;2\/3&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;116206&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;avantages-systeme-hybridomes&#8221;]Recombinant antibody production process[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;102775&#8243; text_color_type=&#8221;uncode-palette&#8221;]From variable-fragment synthesis to the <strong>production of a purified, quality-controlled antibody<\/strong>, we provide a complete, flexible process with a standard turnaround time of five to six weeks.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_single_image media=&#8221;110984&#8243; media_width_percent=&#8221;100&#8243; shape=&#8221;img-round&#8221; radius=&#8221;xl&#8221; uncode_shortcode_id=&#8221;951855&#8243;][\/vc_column_inner][\/vc_row_inner][vc_accordion history=&#8221;yes&#8221; gutter_simple=&#8221;0&#8243; uncode_shortcode_id=&#8221;214352&#8243; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Antibody production by transient transfection&#8221; tab_id=&#8221;1777886430-1-971784795795899&#8243;][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text uncode_shortcode_id=&#8221;176041&#8243;]<\/p>\n<ul>\n<li data-section-id=\"tfjhfo\" data-start=\"5280\" data-end=\"5363\"><strong data-start=\"5282\" data-end=\"5300\">Optional step:<\/strong> sequencing of the monoclonal antibody\u2019s heavy and light chains<\/li>\n<li data-section-id=\"v1xoqu\" data-start=\"5364\" data-end=\"5428\"><strong data-start=\"5366\" data-end=\"5377\">Step 1:<\/strong> gene synthesis of the VH and VL variable fragments<\/li>\n<li data-section-id=\"18ulci9\" data-start=\"5429\" data-end=\"5494\"><strong data-start=\"5431\" data-end=\"5442\">Step 2:<\/strong> cloning of the fragments into the expression vector<\/li>\n<li data-section-id=\"shz7si\" data-start=\"5495\" data-end=\"5554\"><strong data-start=\"5497\" data-end=\"5508\">Step 3:<\/strong> antibody expression by transient transfection<\/li>\n<li data-section-id=\"9nh2n6\" data-start=\"5555\" data-end=\"5615\"><strong data-start=\"5557\" data-end=\"5568\">Step 4:<\/strong> production monitoring using our <a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/fastelisa-kits\/\">FastELISA<\/a> kits<\/li>\n<li data-section-id=\"190f1z1\" data-start=\"5616\" data-end=\"5658\"><strong data-start=\"5618\" data-end=\"5629\">Step 5:<\/strong> chromatographic purification<\/li>\n<li data-section-id=\"1yov4j5\" data-start=\"5659\" data-end=\"5970\"><strong data-start=\"5661\" data-end=\"5672\">Step 6:<\/strong> quality-control testing:\n<ul data-start=\"5700\" data-end=\"5970\">\n<li data-section-id=\"o79825\" data-start=\"5700\" data-end=\"5771\">Purity testing by SDS-PAGE under reducing and non-reducing conditions<\/li>\n<li data-section-id=\"1s68hs0\" data-start=\"5774\" data-end=\"5856\">Total-protein quantification by absorbance measurement using a spectrophotometer<\/li>\n<li data-section-id=\"1774ioe\" data-start=\"5859\" data-end=\"5970\">Optional additional testing, including SEC, LAL assays, Western blotting, ELISA, mass spectrometry and CE-SDS<\/li>\n<\/ul>\n<\/li>\n<li data-section-id=\"8yi9jy\" data-start=\"5971\" data-end=\"6043\"><strong data-start=\"5973\" data-end=\"5984\">Step 7:<\/strong> packaging and shipping, accompanied by a production report<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;684007&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_single_image media=&#8221;91306&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;124590&#8243;][vc_column_text uncode_shortcode_id=&#8221;968494&#8243;]<strong>What are the turnaround times?<\/strong><\/p>\n<ul>\n<li data-section-id=\"hkr487\" data-start=\"6080\" data-end=\"6198\">Two to three weeks for <strong>VH and VL variable-fragment synthesis<\/strong> and <strong>subcloning into the expression vector<\/strong>\u2014steps 1 and 2<\/li>\n<li data-section-id=\"kde6bl\" data-start=\"6199\" data-end=\"6291\">Three weeks for <strong>antibody production, purification and quality-control testing<\/strong>\u2014steps 3 to 6<\/li>\n<li data-section-id=\"13xqje4\" data-start=\"6292\" data-end=\"6400\">Five to six weeks for the complete process, from <strong>gene synthesis to purified, quality-controlled antibodies<\/strong><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Fast-Track production in only four weeks&#8221; tab_id=&#8221;1777886430-2-981784795795899&#8243;][vc_row_inner][vc_column_inner width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;698120&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"6446\" data-end=\"6527\">Is time a critical factor in your project? Do you need your pilot batches sooner?<\/p>\n<p data-start=\"6529\" data-end=\"6627\">Our <strong>Fast-Track production service<\/strong> enables you to receive your antibody batches in only four weeks.<\/p>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Antibody production by stable transfection&#8221; tab_id=&#8221;1777887677769-2-51784795795899&#8243;][vc_row_inner][vc_column_inner width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;212754&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"6675\" data-end=\"6815\">We <strong>produce recombinant antibodies<\/strong> from either a <strong>stable cell line<\/strong> or a s<strong>table cell pool,<\/strong> supplied by the customer or developed by RD-Biotech.<\/p>\n<p data-start=\"6817\" data-end=\"6984\">Widely used in <strong>biotechnology<\/strong>, this approach enables the <strong>production of large antibody quantities <\/strong>(up to several grams) while ensuring consistent, reproducible expression.<\/p>\n<p>[\/vc_column_text][vc_single_image media=&#8221;110985&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;110332&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;583022&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;recombinant-antibody-reformatting&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;601633&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;avantages-systeme-hybridomes&#8221;]Recombinant antibody reformatting[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;40&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;688625&#8243; row_height_pixel=&#8221;400&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;129206&#8243;][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;443663&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"7023\" data-end=\"7215\"><strong>Recombinant antibody reformatting<\/strong> is a key process in <strong>biotechnology and therapeutic research<\/strong>. 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