{"id":109996,"date":"2026-07-22T15:47:26","date_gmt":"2026-07-22T13:47:26","guid":{"rendered":"https:\/\/rd-biotech.tetedecom.pro\/?page_id=109996"},"modified":"2026-07-27T14:37:06","modified_gmt":"2026-07-27T12:37:06","slug":"plasmid-construction","status":"publish","type":"page","link":"https:\/\/rd-biotech.tetedecom.pro\/en\/molecular-biology-and-plasmid-dna\/plasmid-construction\/","title":{"rendered":"Plasmid construction"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;40&#8243; back_image=&#8221;109061&#8243; back_repeat=&#8221;no-repeat&#8221; back_position=&#8221;center center&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;164373&#8243; row_height_pixel=&#8221;400&#8243; overlay_color_type=&#8221;uncode-palette&#8221; el_class=&#8221;header&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;1&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;484880&#8243;][uncode_breadcrumbs][vc_empty_space empty_h=&#8221;2&#8243;][vc_custom_heading auto_text=&#8221;yes&#8221; text_size=&#8221;fontsize-155944&#8243; text_weight=&#8221;200&#8243; text_space=&#8221;fontspace-781688&#8243; uncode_shortcode_id=&#8221;497824&#8243;][\/vc_custom_heading][vc_empty_space empty_h=&#8221;2&#8243;][vc_custom_heading text_size=&#8221;h5&#8243; text_weight=&#8221;200&#8243; css_animation=&#8221;alpha-anim&#8221; animation_speed=&#8221;2000&#8243; uncode_shortcode_id=&#8221;436198&#8243;]<\/p>\n<p data-pm-slice=\"1 1 []\">Every year, RD-Biotech designs and produces more than 1,000 plasmids for over 500 clients (including academic researchers, industrial R&amp;D teams, and biotech companies in clinical development).<\/p>\n<p>[\/vc_custom_heading][vc_accordion label_border=&#8221;yes&#8221; content_border=&#8221;yes&#8221; gutter_simple=&#8221;0&#8243; uncode_shortcode_id=&#8221;207852&#8243; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;0&#8243; column_padding=&#8221;0&#8243; title=&#8221;En savoir +&#8221; tab_id=&#8221;1782132656-1-221784893547827&#8243;][vc_column_text uncode_shortcode_id=&#8221;143142&#8243;]<\/p>\n<p data-pm-slice=\"1 1 []\"><strong>Expression vectors<\/strong>, reporter plasmids, multicistronic constructs, and CRISPR tools: the wide variety of requests we receive reflects the breadth of fields involved, from fundamental cell biology to gene therapy applications.<\/p>\n<p>This is why our approach always begins by listening: understanding your context, current stage, and objectives. Whether you have a finalized design or are still looking for the best strategy, our experts adapt to your needs and recommend the most suitable solution.<\/p>\n<p>Beyond <strong>vector construction<\/strong>, RD-Biotech can support you through to recombinant protein expression\u2014whether you wish to outsource the entire workflow or benefit from in-house functional validation.<\/p>\n<h3><\/h3>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;201360&#8243; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;custom-plasmid&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;817706&#8243; text_color_type=&#8221;uncode-palette&#8221;]Custom Plasmids[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;834390&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p data-pm-slice=\"1 1 []\">The starting point for <strong>plasmid construction<\/strong> is rarely the same from one project to another. That is why we have developed an approach with no prerequisites: you provide whatever material or information you have, and we take care of the rest.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;40&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;314324&#8243; row_height_pixel=&#8221;400&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;203750&#8243; row_height_pixel=&#8221;250&#8243;][vc_column_inner width=&#8221;7\/12&#8243;][vc_column_text uncode_shortcode_id=&#8221;106534&#8243;]<\/p>\n<p data-pm-slice=\"1 1 []\">Starting material may take many forms: a <strong>coding or regulatory nucleic acid sequence<\/strong>, a protein sequence, a <strong>GenBank<\/strong> or <strong>UniProt<\/strong> identifier, or simply a bibliographic reference. In the latter case, our experts identify and retrieve the sequence of interest on your behalf.<\/p>\n<p>Several vector options are available: we can select the backbone best suited to your expression system from our in-house collection, or work with your own acceptor vector under strict confidentiality. You may also provide us with a plasmid sequence that you do not physically possess, and we will reconstruct it.<\/p>\n<p>The diversity of the constructs we produce\u2014including large inserts, high-GC sequences, repeated elements, and toxic fragments\u2014demonstrates our command of a broad range of <strong>cloning strategies<\/strong>, selected and adapted to the specific requirements of each project.[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;111165&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;5\/12&#8243; uncode_shortcode_id=&#8221;209664&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;accent&#8221; overlay_alpha=&#8221;0&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;756429&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;143731&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h3&#8243; text_size=&#8221;h3&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;187030&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;Bioproduction-de-plasmides-quel-grade-pour-mon-application?&#8221;]What you provide <b>\u2193<\/b>[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;756984&#8243; back_color_type=&#8221;uncode-solid&#8221; back_color_solid=&#8221;#f0fbff&#8221;][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-link2&#8243; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;544092&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Nucleic acid or protein sequence&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-flask&#8221; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;166606&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Your own vector&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-puzzle-piece&#8221; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;497991&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Complex construct&#8221; el_class=&#8221;box-avec-tooltip&#8221;]Texte de description au survol.[\/vc_icon][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; 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size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;344668&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Gene library&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon_image=&#8221;110586&#8243; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;902654&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Viral vectors (AAV, lentivirus)&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-cogs&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;242404&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Cell line development&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;138479&#8243; back_color_type=&#8221;uncode-solid&#8221; back_color_solid=&#8221;#f0fbff&#8221;][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-scissors&#8221; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;200283&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;CRISPR \/ Cas9&#8243; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-bar-chart&#8221; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;419512&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;PCR controls and reporters&#8221; el_class=&#8221;box-avec-tooltip&#8221;]Texte de description au survol.[\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-hourglass-2&#8243; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;913137&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Recombinant protein expression&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;185098&#8243; back_color_type=&#8221;uncode-solid&#8221; back_color_solid=&#8221;#f0fbff&#8221;][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-pencil3&#8243; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;919216&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Research tools&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon_image=&#8221;110585&#8243; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;133232&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;shNRA \/ miRNA&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-stumbleupon&#8221; icon_color=&#8221;color-109923&#8243; size=&#8221;fa-2x&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;108043&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;ARNm \/ Transcription IVT&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;accent&#8221; overlay_alpha=&#8221;0&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;899531&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;143731&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h3&#8243; text_size=&#8221;h3&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;112064&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;Bioproduction-de-plasmides-quel-grade-pour-mon-application?&#8221;]Deliverables[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;267927&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p data-pm-slice=\"1 1 []\">All<strong> vectors cloned<\/strong> by RD-Biotech come with a 100% <strong>sequence-fidelity guarantee<\/strong>. The quality-control checks applied to custom vector cloning are listed below.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;189842&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_tabs tab_scrolling=&#8221;yes&#8221; uncode_shortcode_id=&#8221;605913&#8243; el_class=&#8221;tab-services mobile-tabs-to-dropdown&#8221;][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Plasmid&#8221; tab_id=&#8221;1757666499-1-6177753499153529361784893548445&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;0&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;955701&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;0&#8243; border_color=&#8221;transparent&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;472868&#8243; el_class=&#8221;service-box&#8221; border_color_type=&#8221;uncode-palette&#8221; css=&#8221;.vc_custom_1777536877602{border-top-width: 0px !important;}&#8221;][vc_column_text uncode_shortcode_id=&#8221;207164&#8243;]<\/p>\n<p data-pm-slice=\"1 3 []\">Spectrophotometry with optical density (OD) measurement at 260\/280 nm<\/p>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Plasmid map&#8221; tab_id=&#8221;1757666499-2-86177753499153568721784893548445&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;256557&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;334868&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;283914&#8243;]<\/p>\n<ul>\n<li style=\"text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">N.A<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Cloning report&#8221; tab_id=&#8221;1782380537222-2-101784893548445&#8243;][vc_row_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;981184&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;125133&#8243;]<\/p>\n<p data-pm-slice=\"1 3 []\"><strong>Agarose gel electrophoresis<\/strong><\/p>\n<ul data-spread=\"false\">\n<li>Undigested profile<\/li>\n<li>Restriction digest profile<\/li>\n<\/ul>\n<p><strong>Polyacrylamide gel electrophoresis<\/strong><\/p>\n<ul data-spread=\"false\">\n<li>Poly(A) digestion profile, where applicable<\/li>\n<li>Other quality-control tests available on request<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Sequencing report&#8221; tab_id=&#8221;1782380561121-3-101784893548445&#8243;][vc_row_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;149306&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;201618&#8243;]<\/p>\n<ul data-spread=\"false\" data-pm-slice=\"3 3 []\">\n<li>Sanger sequencing of the insert<\/li>\n<li>Complete plasmid sequencing using Oxford Nanopore technology<\/li>\n<li>Complete plasmid sequencing by Sanger, on request<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tab][\/vc_tabs][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;444346&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;plasmids-for-antibody-and-recombinant-protein&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;149796&#8243; text_color_type=&#8221;uncode-palette&#8221;]Plasmids for antibody and recombinant protein[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;192745&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p data-pm-slice=\"1 1 []\">The <strong>performance of a recombinant protein<\/strong> or <strong>antibody<\/strong> depends on the <strong>quality of the expression vector<\/strong> used. RD-Biotech supports you from the earliest stages of your project by <strong>designing plasmids tailored<\/strong> to your biological constraints, expression objectives, and end applications.<\/p>\n<p>Whether you are working with <strong>antibodies in any format or with complex recombinant proteins<\/strong>, we optimize every component of the plasmid (including the sequence, promoters, tags, and secretion signals) to maximize product yield, solubility, and functionality.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;139686&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;118103&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h3&#8243; text_size=&#8221;h3&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;219901&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;Bioproduction-de-plasmides-quel-grade-pour-mon-application?&#8221;]Antibodies[\/vc_custom_heading][vc_column_text text_color=&#8221;color-wvjs&#8221; uncode_shortcode_id=&#8221;660720&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p style=\"text-align: center;\" data-pm-slice=\"1 1 []\">All formats \u00b7 All species \u00b7 All isotypes<\/p>\n<p>[\/vc_column_text][vc_column_text text_color=&#8221;color-193705&#8243; uncode_shortcode_id=&#8221;235627&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p style=\"text-align: center;\">What you provide<\/p>\n<p>[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;902188&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h3&#8243; text_size=&#8221;h3&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;136722&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;Bioproduction-de-plasmides-quel-grade-pour-mon-application?&#8221;]Recombinant proteins[\/vc_custom_heading][vc_column_text text_color=&#8221;color-wvjs&#8221; uncode_shortcode_id=&#8221;121207&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p style=\"text-align: center;\" data-pm-slice=\"1 1 []\">All protein types \u00b7 All expression systems<\/p>\n<p>[\/vc_column_text][vc_column_text text_color=&#8221;color-193705&#8243; uncode_shortcode_id=&#8221;144488&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p style=\"text-align: center;\">What you provide<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;1&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;148417&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;bottom&#8221; gutter_size=&#8221;0&#8243; override_padding=&#8221;yes&#8221; 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icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Host-specific codon optimization&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-tag&#8221; icon_color=&#8221;color-xsdn&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;553852&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Tag selection and design (His, GST, FLAG, etc.)&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-command&#8221; icon_color=&#8221;color-xsdn&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;140987&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Selection of the appropriate expression vector&#8221; el_class=&#8221;box-avec-tooltip&#8221;]Texte de description au survol.[\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-th&#8221; icon_color=&#8221;color-xsdn&#8221; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;917290&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Selection of the expression system&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;0&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;533599&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;0&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;0&#8243; expand_height=&#8221;yes&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;830523&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_icon position=&#8221;bottom&#8221; icon=&#8221;fa fa-chevron-down&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; text_weight=&#8221;600&#8243; uncode_shortcode_id=&#8221;430715&#8243; icon_color_type=&#8221;uncode-palette&#8221; el_class=&#8221;box-avec-tooltip&#8221;]Texte de description au survol.[\/vc_icon][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;0&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;0&#8243; expand_height=&#8221;yes&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;119856&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_icon position=&#8221;bottom&#8221; icon=&#8221;fa fa-chevron-down&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; text_weight=&#8221;600&#8243; uncode_shortcode_id=&#8221;126701&#8243; icon_color_type=&#8221;uncode-palette&#8221; el_class=&#8221;box-avec-tooltip&#8221;]Texte de description au survol.[\/vc_icon][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;1&#8243; bottom_padding=&#8221;1&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; 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el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-check&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;278044&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Full-length IgG, Fab, F(ab\u2019)\u2082, scFv&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-check&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;181611&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Bispecific antibodies, VHH&#8221; el_class=&#8221;box-avec-tooltip&#8221;]Texte de description au survol.[\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-check&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;504568&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;All isotypes, all species&#8221; el_class=&#8221;box-avec-tooltip&#8221;]Texte de description au survol.[\/vc_icon][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;bottom&#8221; gutter_size=&#8221;0&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;805787&#8243; back_color_type=&#8221;uncode-solid&#8221; back_color_solid=&#8221;#ebf6fa&#8221;][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-chevron-circle-down&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; text_weight=&#8221;600&#8243; uncode_shortcode_id=&#8221;207960&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Protein types&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-check&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;498596&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Membrane proteins, enzymes, cytokines&#8221; el_class=&#8221;box-avec-tooltip&#8221;]<\/p>\n<p data-pm-slice=\"1 1 [&quot;list&quot;,{&quot;spread&quot;:false,&quot;start&quot;:16085,&quot;end&quot;:16191},&quot;regular_list_item&quot;,{&quot;start&quot;:16085,&quot;end&quot;:16124}]\">Membrane proteins, enzymes, cytokines<\/p>\n<p>[\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-check&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;190317&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Growth factors&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][vc_icon position=&#8221;left&#8221; space_reduced=&#8221;yes&#8221; icon=&#8221;fa fa-check&#8221; icon_color=&#8221;color-109923&#8243; heading_semantic=&#8221;div&#8221; text_size=&#8221;h5&#8243; uncode_shortcode_id=&#8221;203880&#8243; icon_color_type=&#8221;uncode-palette&#8221; title=&#8221;Fusion proteins, reporters, structural proteins&#8221; el_class=&#8221;box-avec-tooltip&#8221;][\/vc_icon][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;4&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;153178&#8243; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;plasmides-pour-la-production-daRNm-par-transcription-in-vitro&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h3&#8243; text_size=&#8221;h3&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;686243&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;Bioproduction-de-plasmides-quel-grade-pour-mon-application?&#8221;]Tow operating models to meet your requirements[\/vc_custom_heading][vc_column_text text_color=&#8221;accent&#8221; uncode_shortcode_id=&#8221;440598&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p data-pm-slice=\"1 1 []\">RD-Biotech adapts to your intellectual property requirements. For performance-driven projects, we use licensed technology that delivers <strong>transient antibody-expression<\/strong> yields of up to 1 g\/L; in this model, the plasmid material remains the property of RD-Biotech.<\/p>\n<p>For projects requiring technology transfer, we work with royalty-free vectors that are made fully available to the client upon project completion.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;accent&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;204682&#8243; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;plasmides-for-mRNA-production-by-in-vitro-transcription&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;185907&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;111565&#8243; text_color_type=&#8221;uncode-palette&#8221;]Plasmids for mRNA production by <em>in vitro <\/em>transcription[\/vc_custom_heading][vc_column_text text_color=&#8221;color-xsdn&#8221; uncode_shortcode_id=&#8221;539576&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p data-pm-slice=\"1 1 []\">The quality of m<strong>RNA produced by <em>in vitro t<\/em>ranscription<\/strong> depends first and foremost on the quality of its plasmid template. The transcription promoter, 5\u2032 and 3\u2032 untranslated regions (UTRs), open reading frame (ORF), poly(A) tail, and linearization site all directly influence transcription yield, <em>mRNA stability<\/em>, and translation efficiency.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;accent&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;594267&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;bottom&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;0&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; expand_height=&#8221;yes&#8221; style=&#8221;dark&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;7\/12&#8243; uncode_shortcode_id=&#8221;377299&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_row_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;145765&#8243;][vc_column_text text_color=&#8221;accent&#8221; uncode_shortcode_id=&#8221;179340&#8243; text_color_type=&#8221;uncode-palette&#8221;]RD-Biotech can contribute at every stage of the design process. We can <strong>modify an existing plasmid<\/strong> by:<\/p>\n<ul data-spread=\"false\">\n<li>changing the ORF, optimizing the UTRs, adjusting poly(A)-tail length, and repositioning the linearization site; or<\/li>\n<li>building a new template from royalty-free vectors into which we incorporate royalty-free UTRs combined with the ORF of your choice.<\/li>\n<\/ul>\n<p><strong>Plasmid linearization<\/strong> can also be performed in-house, as can the production of small IVT mRNA batches for proof-of-concept (POC) validation.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; border_color=&#8221;color-117289&#8243; border_style=&#8221;solid&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;5\/12&#8243; uncode_shortcode_id=&#8221;627991&#8243; css=&#8221;.vc_custom_1784185846904{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;padding-top: 30px !important;}&#8221; border_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_single_image media=&#8221;111163&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; shape=&#8221;img-round&#8221; radius=&#8221;hg&#8221; uncode_shortcode_id=&#8221;128634&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;4&#8243; bottom_padding=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;102933&#8243; back_color_type=&#8221;uncode-solid&#8221; back_color_solid=&#8221;#f0fbff&#8221; el_id=&#8221;plasmides-for-viral-vector-production-and-gene-therapy&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;109212&#8243; text_color_type=&#8221;uncode-palette&#8221;]Plasmids for viral vector production and gene therapy[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;150633&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p data-pm-slice=\"1 1 []\"><strong>Viral vector production<\/strong> relies on a <strong>multiple-plasmid system<\/strong> whose quality and design directly determine the performance of the final vector. RD-Biotech has expertise in constructing all of these components\u2014<strong>helper, packaging, and transfer plasmids<\/strong>\u2014for the main systems used in gene therapy, primarily <strong>AAV<\/strong> and <strong>lentiviral vectors<\/strong>, with other systems available on request.<\/p>\n<p>[\/vc_column_text][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;129206&#8243;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;131443&#8243;][vc_column_text uncode_shortcode_id=&#8221;147106&#8243;]The <strong>transfer plasmid<\/strong> lies at the heart of our <strong>molecular biology<\/strong> work. It carries the transgene of interest and its associated regulatory elements\u2014including the promoter, ITRs, packaging signals, and insulator elements. 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media_width_percent=&#8221;100&#8243; media_ratio=&#8221;four-three&#8221; shape=&#8221;img-round&#8221; radius=&#8221;lg&#8221; uncode_shortcode_id=&#8221;298853&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;155799&#8243; el_class=&#8221;other-services&#8221; el_id=&#8221;nous-contacter&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; 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