{"id":110320,"date":"2026-07-22T15:54:53","date_gmt":"2026-07-22T13:54:53","guid":{"rendered":"https:\/\/rd-biotech.tetedecom.pro\/?page_id=110320"},"modified":"2026-07-27T16:10:19","modified_gmt":"2026-07-27T14:10:19","slug":"process-development-and-scale-up","status":"publish","type":"page","link":"https:\/\/rd-biotech.tetedecom.pro\/en\/recombinant-protein-expression\/process-development-and-scale-up\/","title":{"rendered":"Process development and scale-up"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;40&#8243; back_image=&#8221;109703&#8243; back_repeat=&#8221;no-repeat&#8221; back_position=&#8221;center center&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;140825&#8243; row_height_pixel=&#8221;400&#8243; overlay_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;1&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;484880&#8243;][uncode_breadcrumbs][vc_empty_space empty_h=&#8221;2&#8243;][vc_custom_heading auto_text=&#8221;yes&#8221; text_size=&#8221;fontsize-155944&#8243; text_weight=&#8221;200&#8243; text_space=&#8221;fontspace-781688&#8243; uncode_shortcode_id=&#8221;497824&#8243;][\/vc_custom_heading][vc_empty_space empty_h=&#8221;2&#8243;][vc_custom_heading text_size=&#8221;h5&#8243; text_weight=&#8221;200&#8243; css_animation=&#8221;alpha-anim&#8221; animation_speed=&#8221;2000&#8243; uncode_shortcode_id=&#8221;435202&#8243;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"255\" data-end=\"370\">We purify and analytically characterize your recombinant proteins using optimized methods tailored to each project.<\/p>\n<p data-start=\"372\" data-end=\"499\">Our analyses ensure the purity, integrity and functional quality of your proteins, providing reliable and reproducible results.<\/p>\n<p>[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;401632&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;process-development&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;150278&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;avantages-systeme-hybridomes&#8221;]Process development[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;134136&#8243; text_color_type=&#8221;uncode-palette&#8221;]<strong>Optimize the expression of your protein<\/strong> through a customized process, from bacterial strain selection to the precise adjustment of induction conditions.<\/p>\n<p>The parameters we can optimize include:[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;209176&#8243; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;plasmides-pour-autres-applications&#8221; row_height_pixel=&#8221;400&#8243;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;141311&#8243;][vc_custom_heading heading_semantic=&#8221;div&#8221; text_font=&#8221;font-146233&#8243; text_size=&#8221;h6&#8243; text_weight=&#8221;600&#8243; text_space=&#8221;fontspace-265885&#8243; uncode_shortcode_id=&#8221;125989&#8243;]Bacterial strain[\/vc_custom_heading][vc_separator sep_color=&#8221;&#8221; uncode_shortcode_id=&#8221;187426&#8243;][vc_custom_heading heading_semantic=&#8221;div&#8221; text_font=&#8221;font-146233&#8243; text_size=&#8221;h6&#8243; text_weight=&#8221;600&#8243; text_space=&#8221;fontspace-265885&#8243; uncode_shortcode_id=&#8221;201086&#8243;]Induction temperature and duration[\/vc_custom_heading][vc_separator sep_color=&#8221;&#8221; uncode_shortcode_id=&#8221;187426&#8243;][vc_custom_heading heading_semantic=&#8221;div&#8221; text_font=&#8221;font-146233&#8243; text_size=&#8221;h6&#8243; text_weight=&#8221;600&#8243; text_space=&#8221;fontspace-265885&#8243; uncode_shortcode_id=&#8221;697941&#8243;]Induction system[\/vc_custom_heading][vc_separator sep_color=&#8221;&#8221; uncode_shortcode_id=&#8221;187426&#8243;][vc_custom_heading heading_semantic=&#8221;div&#8221; text_font=&#8221;font-146233&#8243; text_size=&#8221;h6&#8243; text_weight=&#8221;600&#8243; text_space=&#8221;fontspace-265885&#8243; uncode_shortcode_id=&#8221;614497&#8243;]CRISPR guide design[\/vc_custom_heading][vc_separator sep_color=&#8221;&#8221; uncode_shortcode_id=&#8221;187426&#8243;][vc_custom_heading heading_semantic=&#8221;div&#8221; text_font=&#8221;font-146233&#8243; text_size=&#8221;h6&#8243; text_weight=&#8221;600&#8243; text_space=&#8221;fontspace-265885&#8243; uncode_shortcode_id=&#8221;169862&#8243;]Inducer concentration[\/vc_custom_heading][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;89065&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;xl&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243; uncode_shortcode_id=&#8221;630300&#8243;][vc_single_image media_width_percent=&#8221;55&#8243; shape=&#8221;img-round&#8221; radius=&#8221;xl&#8221; uncode_shortcode_id=&#8221;816684&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;184301&#8243; back_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;scale-up-production&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;112341&#8243; text_color_type=&#8221;uncode-palette&#8221;]Scale-up and Production[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;145481&#8243; text_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<p class=\"PDq2pG_selectionAnchorContainer\" data-start=\"870\" data-end=\"945\">We offer a broad range of <strong>purification techniques tailored to your protein<\/strong>:<\/p>\n<ul data-start=\"947\" data-end=\"1145\">\n<li data-section-id=\"145p8da\" data-start=\"947\" data-end=\"972\">Affinity chromatography<\/li>\n<li data-section-id=\"1ppp1xe\" data-start=\"973\" data-end=\"1002\">Ion-exchange chromatography<\/li>\n<li data-section-id=\"1pho75d\" data-start=\"1003\" data-end=\"1043\">Hydrophobic interaction chromatography<\/li>\n<li data-section-id=\"1gszqqw\" data-start=\"1044\" data-end=\"1075\">Size-exclusion chromatography<\/li>\n<li data-section-id=\"1os8w5g\" data-start=\"1076\" data-end=\"1090\">Tag cleavage<\/li>\n<li data-section-id=\"1e3iiw2\" data-start=\"1091\" data-end=\"1110\">Endotoxin removal<\/li>\n<li data-section-id=\"1ba65w7\" data-start=\"1111\" data-end=\"1145\">Other product-specific processes<\/li>\n<\/ul>\n<p data-start=\"1147\" data-end=\"1201\">Every stage is optimized to maximize yield and purity.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;140868&#8243; row_height_pixel=&#8221;400&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;115727&#8243;][vc_tabs tab_scrolling=&#8221;yes&#8221; uncode_shortcode_id=&#8221;605913&#8243; el_class=&#8221;tab-services mobile-tabs-to-dropdown&#8221;][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Upstream&#8221; tab_id=&#8221;1757666499-1-617775349915352931784812204659&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;0&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;989195&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221; row_height_pixel=&#8221;300&#8243;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;0&#8243; border_color=&#8221;transparent&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;2\/3&#8243; uncode_shortcode_id=&#8221;633727&#8243; el_class=&#8221;service-box&#8221; border_color_type=&#8221;uncode-palette&#8221; css=&#8221;.vc_custom_1784290356130{border-top-width: 0px !important;}&#8221;][vc_column_text uncode_shortcode_id=&#8221;191411&#8243;]<\/p>\n<ul class=\"ul1\">\n<li style=\"text-align: left;\" data-section-id=\"x9lqd9\" data-start=\"1228\" data-end=\"1271\">Production in 250 mL to 15 L shake flasks<\/li>\n<li style=\"text-align: left;\" data-section-id=\"1hlbzrf\" data-start=\"1272\" data-end=\"1313\">Production in 250 mL to 10 L fermenters<\/li>\n<li style=\"text-align: left;\" data-section-id=\"h7l9n6\" data-start=\"1314\" data-end=\"1365\">Collection of bacterial pellets by centrifugation<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;111200&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;496884&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Downstream&#8221; tab_id=&#8221;1772808992395-2-317775349915352921784812204659&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;256557&#8243; el_class=&#8221;services-hover vc_custom_preserve_mobile&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;214884&#8243; el_class=&#8221;service-box&#8221;][vc_column_text uncode_shortcode_id=&#8221;275000&#8243;]<\/p>\n<ul>\n<li style=\"text-align: left;\" data-section-id=\"1cro5he\" data-start=\"1394\" data-end=\"1566\">Solubilization of bacterial pellets using sonication, microfluidization, French press processing or various detergents and denaturing agents, including urea and guanidine<\/li>\n<li style=\"text-align: left;\" data-section-id=\"6jitgp\" data-start=\"1567\" data-end=\"1605\">Affinity chromatography using Ni-NTA<\/li>\n<li style=\"text-align: left;\" data-section-id=\"1ppp1xe\" data-start=\"1606\" data-end=\"1635\">Ion-exchange chromatography<\/li>\n<li style=\"text-align: left;\" data-section-id=\"165hp0e\" data-start=\"1636\" data-end=\"1652\">Gel filtration<\/li>\n<li style=\"text-align: left;\" data-section-id=\"19de9qd\" data-start=\"1653\" data-end=\"1672\">Protein refolding<\/li>\n<li style=\"text-align: left;\" data-section-id=\"146dje1\" data-start=\"1673\" data-end=\"1684\">Polishing<\/li>\n<\/ul>\n<p class=\"PDq2pG_selectionAnchorContainer\" style=\"text-align: left;\" data-start=\"1686\" data-end=\"1861\">The harvested culture supernatant can be processed by tangential flow filtration and concentrated up to 20-fold. This step optimizes purification for large production volumes.<\/p>\n<p style=\"text-align: left;\" data-start=\"1863\" data-end=\"1990\">The concentrated culture supernatant can also be supplied as the finished product, with optional diafiltration into PBS buffer.<\/p>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tab][\/vc_tabs][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;0&#8243; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;121547&#8243; row_height_pixel=&#8221;0&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-solid&#8221; el_id=&#8221;advanced-characterization-and-stability&#8221; back_color_solid=&#8221;#f0fbff&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_custom_heading text_color=&#8221;color-109923&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;979804&#8243; text_color_type=&#8221;uncode-palette&#8221; el_id=&#8221;avantages-systeme-hybridomes&#8221;]Advanced characterization and stability[\/vc_custom_heading][vc_column_text text_color=&#8221;color-109923&#8243; uncode_shortcode_id=&#8221;810488&#8243; text_color_type=&#8221;uncode-palette&#8221;]We provide multi-step purification (including affinity, size-exclusion and ion-exchange chromatography) together with <strong>comprehensive characterization of your recombinant proteins<\/strong>.<\/p>\n<p>We ensure high purity and a quality level suited to your research, preclinical or clinical applications.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;4&#8243; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;146529&#8243; overlay_color_type=&#8221;uncode-palette&#8221; back_color_type=&#8221;uncode-solid&#8221; back_color_solid=&#8221;#f0fbff&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_center_mobile&#8221; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;932974&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;102621&#8243;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; expand_height=&#8221;yes&#8221; back_image=&#8221;110922&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;5\/12&#8243; uncode_shortcode_id=&#8221;379492&#8243;][\/vc_column_inner][vc_column_inner width=&#8221;7\/12&#8243;][vc_column_text uncode_shortcode_id=&#8221;206934&#8243;]<\/p>\n<p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" tabindex=\"-1\" role=\"text\" data-placeholder=\"Traduction\" data-ved=\"2ahUKEwiTwtD-6eiVAxWOcvEDHU04IxoQ3ewLegQIDRAV\" aria-label=\"Texte traduit\u00a0: The final quality of your recombinant protein depends on rigorous purification and comprehensive characterization. At RD-Biotech, we have mastered every step of recombinant protein purification, offering the flexibility to precisely meet your application-specific needs. We offer custom purification tailored to each recombinant protein: With any tag: His-tag, GST, FLAG, Strep-tag, Avi-tag, Fc-fusion, or any other tag of your choice, enabling rapid and efficient purification via affinity chromatography. Tag-free: Vector design incorporating an enzymatic cleavage site (TEV, Enterokinase, SUMO, PreScission, etc.), followed by complete cleavage and a separation step to yield a native recombinant protein free of tag residues. This option is particularly valued for structural studies, functional activity assays, and preclinical applications.\"><span class=\"Y2IQFc\" lang=\"en\">The final <strong>quality of your recombinant protein<\/strong> depends on rigorous <strong>purification and comprehensive characterization<\/strong>. <\/span><\/p>\n<p class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" tabindex=\"-1\" role=\"text\" data-placeholder=\"Traduction\" data-ved=\"2ahUKEwiTwtD-6eiVAxWOcvEDHU04IxoQ3ewLegQIDRAV\" aria-label=\"Texte traduit\u00a0: The final quality of your recombinant protein depends on rigorous purification and comprehensive characterization. At RD-Biotech, we have mastered every step of recombinant protein purification, offering the flexibility to precisely meet your application-specific needs. We offer custom purification tailored to each recombinant protein: With any tag: His-tag, GST, FLAG, Strep-tag, Avi-tag, Fc-fusion, or any other tag of your choice, enabling rapid and efficient purification via affinity chromatography. Tag-free: Vector design incorporating an enzymatic cleavage site (TEV, Enterokinase, SUMO, PreScission, etc.), followed by complete cleavage and a separation step to yield a native recombinant protein free of tag residues. This option is particularly valued for structural studies, functional activity assays, and preclinical applications.\"><span class=\"Y2IQFc\" lang=\"en\">At RD-Biotech, we have mastered every step of <strong>recombinant protein purification<\/strong>, offering the flexibility to precisely meet your application-specific needs. <\/span><\/p>\n<ul>\n<li class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" tabindex=\"-1\" role=\"text\" data-placeholder=\"Traduction\" data-ved=\"2ahUKEwiTwtD-6eiVAxWOcvEDHU04IxoQ3ewLegQIDRAV\" aria-label=\"Texte traduit\u00a0: The final quality of your recombinant protein depends on rigorous purification and comprehensive characterization. At RD-Biotech, we have mastered every step of recombinant protein purification, offering the flexibility to precisely meet your application-specific needs. We offer custom purification tailored to each recombinant protein: With any tag: His-tag, GST, FLAG, Strep-tag, Avi-tag, Fc-fusion, or any other tag of your choice, enabling rapid and efficient purification via affinity chromatography. Tag-free: Vector design incorporating an enzymatic cleavage site (TEV, Enterokinase, SUMO, PreScission, etc.), followed by complete cleavage and a separation step to yield a native recombinant protein free of tag residues. This option is particularly valued for structural studies, functional activity assays, and preclinical applications.\"><span class=\"Y2IQFc\" lang=\"en\">We offer custom purification tailored to each recombinant protein: With any tag (His-tag, GST, FLAG, Strep-tag, Avi-tag, Fc-fusion, or any other tag of your choice)\u00a0 enabling <strong>rapid and efficient purification via affinity chromatography<\/strong>.<\/span><\/li>\n<li class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" tabindex=\"-1\" role=\"text\" data-placeholder=\"Traduction\" data-ved=\"2ahUKEwiTwtD-6eiVAxWOcvEDHU04IxoQ3ewLegQIDRAV\" aria-label=\"Texte traduit\u00a0: The final quality of your recombinant protein depends on rigorous purification and comprehensive characterization. At RD-Biotech, we have mastered every step of recombinant protein purification, offering the flexibility to precisely meet your application-specific needs. We offer custom purification tailored to each recombinant protein: With any tag: His-tag, GST, FLAG, Strep-tag, Avi-tag, Fc-fusion, or any other tag of your choice, enabling rapid and efficient purification via affinity chromatography. Tag-free: Vector design incorporating an enzymatic cleavage site (TEV, Enterokinase, SUMO, PreScission, etc.), followed by complete cleavage and a separation step to yield a native recombinant protein free of tag residues. This option is particularly valued for structural studies, functional activity assays, and preclinical applications.\"><span class=\"Y2IQFc\" lang=\"en\">Tag-free: Vector design incorporating an enzymatic cleavage site (TEV, Enterokinase, SUMO, PreScission, etc.), followed by complete cleavage and a separation step to yield a <strong>native recombinant protein free of tag residues<\/strong>. This option is particularly valued for structural studies, functional activity assays, and preclinical applications.<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;155799&#8243; el_class=&#8221;other-services&#8221; el_id=&#8221;nous-contacter&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; radius=&#8221;hg&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;165097&#8243;][vc_custom_heading text_color=&#8221;color-193705&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;400&#8243; uncode_shortcode_id=&#8221;157839&#8243; text_color_type=&#8221;uncode-palette&#8221;]Discover our other recombinant protein expression services[\/vc_custom_heading][uncode_index el_id=&#8221;index-138264&#8243; index_type=&#8221;carousel&#8221; loop=&#8221;size:10|order_by:date|post_type:page|tax_query:108|taxonomy_count:10&#8243; carousel_lg=&#8221;3&#8243; carousel_md=&#8221;3&#8243; carousel_sm=&#8221;1&#8243; gutter_size=&#8221;3&#8243; inner_padding=&#8221;yes&#8221; page_items=&#8221;spacer|two,title|-,text|excerpt,link|default|default_size|scale_mobile|D\u00e9couvrir,spacer_two|half&#8221; carousel_height=&#8221;equal&#8221; carousel_interval=&#8221;3000&#8243; carousel_navspeed=&#8221;400&#8243; carousel_dots=&#8221;yes&#8221; carousel_dots_mobile=&#8221;yes&#8221; stage_padding=&#8221;0&#8243; single_back_color=&#8221;color-xsdn&#8221; single_overlay_opacity=&#8221;1&#8243; single_h_align=&#8221;center&#8221; single_padding=&#8221;2&#8243; single_title_family=&#8221;font-379643&#8243; single_title_dimension=&#8221;h3&#8243; single_title_weight=&#8221;500&#8243; single_text_lead=&#8221;small&#8221; single_border=&#8221;yes&#8221; uncode_shortcode_id=&#8221;744149&#8243; single_icon=&#8221;fa fa-arrow-right2&#8243;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row row_height_percent=&#8221;40&#8243; back_image=&#8221;109703&#8243; back_repeat=&#8221;no-repeat&#8221; back_position=&#8221;center center&#8221; overlay_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;70&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; content_parallax=&#8221;0&#8243; uncode_shortcode_id=&#8221;140825&#8243; row_height_pixel=&#8221;400&#8243; overlay_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_use_pixel=&#8221;yes&#8221; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;1&#8243; [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":110922,"parent":90492,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-110320","page","type-page","status-publish","has-post-thumbnail","hentry","page_category-expression-de-proteines-recombinantes-en"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>RD-Biotech, cloning and pDNA production services, research, High Quality &amp; GMP grades<\/title>\n<meta name=\"description\" content=\"A complete range of custom services, from vector construction to plasmid DNA biomanufacturing, ascalable process from R&amp;D to GMP grades. 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