{"id":90427,"date":"2021-03-25T12:05:46","date_gmt":"2021-03-25T11:05:46","guid":{"rendered":"https:\/\/rd-biotech.tetedecom.pro\/anticorps-monoclonaux\/"},"modified":"2025-04-24T09:26:25","modified_gmt":"2025-04-24T07:26:25","slug":"monoclonal-antibodies","status":"publish","type":"page","link":"https:\/\/rd-biotech.tetedecom.pro\/en\/monoclonal-antibodies\/","title":{"rendered":"Monoclonal antibodies"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row unlock_row=&#8221;&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-4&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; shadow=&#8221;std&#8221; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;2&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;107504&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;6&#8243; mobile_width=&#8221;7&#8243; width=&#8221;5\/6&#8243; uncode_shortcode_id=&#8221;115501&#8243;][vc_custom_heading heading_semantic=&#8221;p&#8221; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;856731&#8243; text_color_type=&#8221;uncode-solid&#8221; text_color_solid=&#8221;#f29402&#8243;]<span style=\"font-weight: 400;\">RD-Biotech\u2019s antibody engineering platform offers a complete range of custom services.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">By contacting RD-Biotech, you will:<\/span><\/p>\n<ul>\n<li>save time while devoting yourself to the major areas of your high added value research,<\/li>\n<li>benefit from our scientific and technical expertise for the production of monoclonal antibodies or the development of your immunoassays.<\/li>\n<\/ul>\n<p>[\/vc_custom_heading][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;2&#8243; mobile_width=&#8221;7&#8243; width=&#8221;1\/6&#8243; uncode_shortcode_id=&#8221;747647&#8243;][vc_single_image media=&#8221;99635&#8243; media_width_percent=&#8221;80&#8243; alignment=&#8221;center&#8221; uncode_shortcode_id=&#8221;105834&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_speed=&#8221;1000&#8243; width=&#8221;1\/1&#8243;]\n\t\t<div id=\"tdc_summary_946329\" class=\"tdc_summary text-lead wpb_content_element \">\n\n\t\t\t<div class=\"tdc_summary_title\">\n\t\t\t\t<a href=\"#\" class=\"summaryAnchor\">Summary<i class=\"fa fa-chevron-down close\" aria-hidden=\"true\"><\/i><\/a>\n\t\t\t<\/div>\n\n\t\t<\/div>\n\t\t[\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;138826&#8243; row_name=&#8221;Monoclonal antibody production&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;7&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;&#8221; overlay_alpha=&#8221;&#8221; shift_x=&#8221;&#8221; shift_y=&#8221;&#8221; shift_y_down=&#8221;&#8221; z_index=&#8221;&#8221; medium_width=&#8221;3&#8243; mobile_width=&#8221;&#8221; css_animation=&#8221;left-t-right&#8221; width=&#8221;7\/12&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Monoclonal antibody production&#8221; el_class=&#8221;icon_box_with_text&#8221;][\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;5&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;5\/12&#8243;][vc_column_text uncode_shortcode_id=&#8221;212345&#8243;]Monoclonal antibodies against murine, rat, guinea pig, or other species. Capacities of up to several tens of grams.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;zoom-out&#8221; width=&#8221;1\/1&#8243;][vc_gallery el_id=&#8221;gallery-639550&#8243; type=&#8221;carousel&#8221; medias=&#8221;99678,88261,88259&#8243; carousel_lg=&#8221;3&#8243; carousel_md=&#8221;1&#8243; carousel_sm=&#8221;1&#8243; thumb_size=&#8221;five-four&#8221; gutter_size=&#8221;3&#8243; carousel_interval=&#8221;3000&#8243; carousel_navspeed=&#8221;400&#8243; stage_padding=&#8221;0&#8243; single_overlay_opacity=&#8221;50&#8243; single_h_align=&#8221;center&#8221; single_v_position=&#8221;bottom&#8221; single_padding=&#8221;2&#8243; single_border=&#8221;yes&#8221; uncode_shortcode_id=&#8221;135762&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243;]RD-Biotech has developed expert know-how in the development and <em>in vitro<\/em> production of monoclonal antibodies.[\/vc_custom_heading][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text]Ascites production, which is still authorized in France, is acknowledged as being very painful for animals and can now be advantageously replaced by cell culture. With its <em>in vitro<\/em> production unit, RD-Biotech offers not only alternative technologies at an equivalent cost to ascites production, but also better reproducibility and higher purity quality compared to ascites production.<\/p>\n<p>When the customer entrusts his clone to RD-Biotech (in flasks or cryotubes) for <em>in vitro<\/em> production, it is not necessary to specify the clone\u2019s specificity, thus providing a guarantee towards customer <strong>confidentiality<\/strong>.<\/p>\n<p>With more than<strong> 500 clones produced<\/strong> each year, RD-Biotech has developed unparalleled expertise in the <strong>production of monoclonal antibodies<\/strong>, for research applications or preclinical studies.<\/p>\n<p>Whether you want 1 mg of purified antibody for an R&amp;D study or 500 mg &#8220;endofree&#8221; for<em> in vivo<\/em> applications, our goal is to meet your demands, in terms of quantity, quality and deadline.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fwp-content%2Fuploads%2F2023%2F05%2FRD-Biotech-mAB-biomanufacturing-services.jpg|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;188023&#8243;]Find out more[\/vc_button][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;500&#8243;]Several quality grades, adapted to your applications:[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-gyho&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]\u201cEndofree\u201d Research &amp; Development grade[\/vc_custom_heading][vc_custom_heading text_color=&#8221;color-193705&#8243; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;400&#8243;]For your R&amp;D and preclinical applications.[\/vc_custom_heading][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Presentation&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517162383315042116238333175451623833669088&#8243; slug=&#8221;presentation&#8221;][vc_column_text]<\/p>\n<ul>\n<li>Large production capacity: from a few milligrams up to several grams. <strong>QUANTITY GUARANTEED!<\/strong><\/li>\n<li>Hybridoma of mice, rats, guinea pigs, or other species<\/li>\n<li>Very short deadlines<\/li>\n<li>Homogeneity of the batches produced<\/li>\n<li>Possibility of secure storage for recurring productions<\/li>\n<li>Adaptation of the process in the event of low \/ bad producing clones<\/li>\n<li>Standard quality controls included, without additional cost: Determination of total proteins by spectrophotometer measurement (Ig standard) and analysis of total proteins by SDS-PAGE electrophoresis.<\/li>\n<li>\u201cEndofree\u201d quality (for<em> in vivo<\/em> applications): We guarantee an endotoxin level of less than 1 EU \/ mg of Ig (use of pyrogen-free buffers, harvest in sterile conditions, sanitization of equipment, pyrogen-free purification conditions, LAL test)<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Related services&#8221; tab_id=&#8221;6dad8f3c-7309-816179609851851623767876517162383315042116238333175451623833669088&#8243; slug=&#8221;related-services:&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Adaptation to a specific and appropriate culture medium<\/li>\n<li aria-level=\"1\">Concentration of the supernatant<\/li>\n<li>Adjustment of antibody concentration<\/li>\n<li>Filtration<\/li>\n<li>Aliquoting<\/li>\n<li>A full range of quality controls available on demand<\/li>\n<li>Controls of specificity, of biological activity&#8230;<\/li>\n<li>Modification of antibodies: fragmentation, coupling,<\/li>\n<li>VH\/VL Sequencing to secure the antibody or to switch to a recombinant mAb production<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Examples of applications&#8221; tab_id=&#8221;1623833716180-2-8&#8243; slug=&#8221;examples-of-applications&#8221;][vc_column_text]<\/p>\n<ul>\n<li>Integration into an in vitro diagnostic kit<\/li>\n<li>Research use<\/li>\n<li>Candidates in a therapeutic program framework<\/li>\n<li>Vaccine controls<\/li>\n<li>Biologically active antibodies<\/li>\n<li>Reference standards<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;6&#8243; back_image=&#8221;99670&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;718603&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;99672&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;201844&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]High Quality Grade (GMP \u201clike\u201d grade)[\/vc_custom_heading][vc_column_text]Production of superior quality monoclonal antibodies, suitable for preclinical applications.<\/p>\n<ul>\n<li>Full traceability and documentation regarding reagents and operators<\/li>\n<li>Large-scale culture performed without antibiotics<\/li>\n<li>The production process is carried out &#8220;animal free&#8221; &#8211; without substances of animal origin<\/li>\n<li>Storage of the cell bank in a secured area<\/li>\n<li>Additional controls available to ensure high purity of the product<\/li>\n<li>Quality controls adapted to the specifications of your project (list of QCs upon request)<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fcontact%2F|title:Contact&#8221;]Find out more[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Monoclonal antibody <em>in vitro<\/em> production[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;193799&#8243;]The different stages of an <em>in vitro<\/em> antibody production program are as follows:<\/p>\n<p>The customer provides the hybridoma (frozen cells or cells in culture) accompanied by a <strong><a href=\"https:\/\/rd-biotech.tetedecom.pro\/wp-content\/uploads\/2021\/07\/Mab-Prod-in-vitro-Technical-data.pdf\" target=\"_blank\" rel=\"noopener\">technical sheet<\/a><\/strong> in which the culture medium, the nature and the isotype of the antibody will be specified. The hybridoma can be adapted to different culture media (serum-free medium, depleted serum in bovine IgG, etc.). The antibody can be produced and purified in Endotoxin free conditions.[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;In vitro production&#8221; tab_id=&#8221;d21d5937-3bb0-2161796098518516237678765171623833150421162383331754516238336690881623833792957&#8243; slug=&#8221;in-vitro-production&#8221;][vc_column_text uncode_shortcode_id=&#8221;201331&#8243;]For the production of large quantities of monoclonal antibodies (over 100 mg) RD-Biotech can carry out a productivity assessment on small volumes (pilot step). At the end of this feasibility phase, the customer receives a report indicating the productivity and an adaptated quotation for the production phase. The customer may also receive the antibody for evaluation before ordering the<em> in vitro<\/em> production phase.<\/p>\n<p><strong>RD-Biotech\u2019s scale up ranges<\/strong> from 1 liter to more than 300 liters of culture supernatant, which yields between a few mg and several tens of grams of antibody.<\/p>\n<p><strong>Different production technologies<\/strong> are available, allowing the cultivation conditions to be adapted to the clone (depending on its productivity, the desired quantity, etc.). Production in Rollers, T-Flasks, possibility to produce in FiberCell, Spinner flasks or CELLine tm.[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Production follow-up&#8221; tab_id=&#8221;6dad8f3c-7309-8161796098518516237678765171623833150421162383331754516238336690881623833792957&#8243; slug=&#8221;production-follow-up&#8221;][vc_column_text]Thanks to the assay kits for mouse IgG, rat IgG and human IgG developed and marketed by RD-Biotech (FastELISA range) , we can monitor the antibody production in the culture supernatant in order to estimate very quickly the amount of supernatant to produce the targeted quantity.[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Ffastelisa-kits%2F&#8221;]DISCOVER FastELISA[\/vc_button][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Concentration of the culture supernatant&#8221; tab_id=&#8221;1623833716180-2-81623833792957&#8243; slug=&#8221;concentration-of-the-culture-supernatant&#8221;][vc_column_text]The supernatant produced can then be concentrated up to 15 times by tangential ultrafiltration.<\/p>\n<p>The supernatant can then either be delivered to the customer or RD-Biotech can carry out the purification of the monoclonal antibody.[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fwp-content%2Fuploads%2F2021%2F07%2FMonoclonal-antibody-in-vitro-production-service-scaled.jpg|target:_blank&#8221;]discover the monoclonal antibody in vitro production service[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88462&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;99674&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;454317&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-142237&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;8\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Antibody purification[\/vc_custom_heading][vc_column_text]<strong>Purification of monoclonal antibodies (Immunoglobulins IgG, IgM, IgA, IgY\u2026):<\/strong><br \/>\n<strong>Purification from serum, plasma, culture supernatant or egg yolk.<\/strong><\/p>\n<p>Using the <strong>FastELISA<\/strong> technique for the quantification of mouse IgG, rat IgG or human IgG, RD-Biotech can rapidly monitor the level of immunoglobulin G in the sample and estimate the amount that it will be possible to purify.[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Ffastelisa-kits%2F&#8221;]DISCOVER FastELISA[\/vc_button][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;RD-Biotech carries out the purification of the antibodies using robust methods which ensure an excellent quality of the purified antibodies.&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545162383366908816238337929571625580582259&#8243; slug=&#8221;rd-biotech-offer&#8221;][vc_column_text]<\/p>\n<ul>\n<li>Purification of monoclonal antibodies by <strong>affinity chromatography<\/strong> on Protein A, Protein G, or other affinity resin, depending on the origin and isotype of the clone (IgG, IgA, etc.)<\/li>\n<li>Purification by <strong>ion exchange chromatography<\/strong>, in particular for the purification of IgM<\/li>\n<li>Purification by<strong> specific affinity<\/strong>: allowing isolation from a specific ligand (peptide, antigen, etc.)<\/li>\n<li>Purification by <strong>precipitation<\/strong>, for example with ammonium sulphate<\/li>\n<li>Purification by <strong>gel filtration<\/strong><\/li>\n<\/ul>\n<p>After the affinity chromatography step, the purity of the antibody can be improved by different methods:<\/p>\n<ul>\n<li>By size exclusion chromatography (<strong>preparative SEC<\/strong>), a step which will remove the aggregates;<\/li>\n<li>With an <strong>ion exchange chromatography<\/strong> step, which also removes any endotoxins that may be present.<\/li>\n<\/ul>\n<p>Antibody produced <em>in vitro<\/em> can be purified in \u201cEndofree\u201d conditions, this requires the use of non-pyrogenic buffers and containers. The monoclonal antibodies produced <em>in vitro<\/em> by RD-Biotech in \u201cEndofree\u201d conditions have an <strong>endotoxin level of less than 1 EU \/ mg.<\/strong>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; radius=&#8221;std&#8221; shadow=&#8221;sm&#8221; width=&#8221;8\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Quality controls[\/vc_custom_heading][vc_column_text]The standard package includes the following controls which guarantee the quality of the antibodies produced and \/ or purified<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Analysis of total proteins by SDS-PAGE electrophoresis<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Determination of total proteins by absorbance measurement with a spectrophotometer (Ig standard adapted to the isotype)<\/li>\n<\/ul>\n<p>Monoclonal antibodies produced <em>in vitro<\/em> can be delivered to the customer after concentration and in the desired buffer, along with a complete purification report.[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Additional controls&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517162383315042116238333175451623833669088162383379295716255805822591625580860128&#8243; slug=&#8221;additional-controls:&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Mycoplasma test<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">LAL test to determine the level of bacterial endotoxins. The LAL (Limulus Amebocyte Lysate) test is performed using a regulatory kit according to the supplier&#8217;s protocol<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Bacterial and fungal contamination test<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Control by analytical SEC<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Desalting of antibodies<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Control of specificity, biological activity &#8230; (ELISA, Western Blotting, Octet, Cytometry, custom bioassays &#8230;)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Determination of Protein A residues\u00a0<\/span><span style=\"font-weight: 400;\">(<\/span><a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/fastelisa-kits\/\"><span style=\"font-weight: 400;\">RD-Biotech FastELISA<\/span><\/a><span style=\"font-weight: 400;\">)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Determination of bovine immunoglobulin contaminants <\/span><span style=\"font-weight: 400;\">(<\/span><a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/fastelisa-kits\/\"><span style=\"font-weight: 400;\">RD-Biotech FastELISA)<\/span><\/a><\/li>\n<\/ul>\n<p>Specific controls will be applied in the case of a &#8220;High Quality Grade&#8221; purification process.<\/p>\n<p>FIND OUT MORE : <a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/contact\/\">contact<\/a><a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/contact\/\"> us<\/a> ![\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fanalytical-services%2F&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;280256&#8243;]DISCOVER ANALYTICAL SERVICES[\/vc_button][vc_button button_color=&#8221;color-109923&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Ffastelisa-kits%2F&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;194359&#8243;]DISCOVER FastELISA[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;99676&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;4\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;117026&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88468&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;8\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Cell banks[\/vc_custom_heading][vc_column_text]Master cell bank and working cell banks<br \/>\nCustom backup banks of your hybridoma:<\/p>\n<ul>\n<li>from cells supplied in culture or frozen<\/li>\n<li>during the cell amplification step before production<\/li>\n<li>or at the end of the clone development process.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;The procedure is as follows:&#8221; tab_id=&#8221;d21d5937-3bb0-2161796098518516237678765171623833150421162383331754516238336690881623833792957162558058225916255808601281625580979981&#8243; slug=&#8221;procedure-is-as-follows&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Cell amplification<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">If necessary, perform a productivity test with the FastELISA Kit.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Freezing of cells in exponential growth step with 100% viability: 10 vials of 5 million cells<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Bank controls (cell viability and absence of contamination by mycoplasmas)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Freezing of the cryotubes at -80 \u00b0 C and in liquid nitrogen before shipment to the customer, accompanied by quality control certification.<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Possibility of secure storage for recurring productions:&#8221; tab_id=&#8221;1626440371905-1-4&#8243; slug=&#8221;possibility-of-secure-storage&#8221;][vc_column_text]<\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Storage in liquid nitrogen (controlled zone)<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Bank stability controls during the storage (productivity and specificity)<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fcell-culture%2F&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;747793&#8243;]DISCOVER CELL CULTURE[\/vc_button][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Ffastelisa-kits%2F|title:ELISA%20kits&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;156247&#8243;]Discover FastELISA[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Antibody coupling[\/vc_custom_heading][vc_column_text]<strong>Coupling, Fragmentation and other modifications:<\/strong><\/p>\n<p>RD-Biotech carries out the fragmentation of the monoclonal antibody or the polyclonal antibody (pepsin, papain, etc.) and \/ or the coupling of the antibodies with different markers:<\/p>\n<ul>\n<li>Peroxidase labelling (HRP)<\/li>\n<li>Alkaline phosphatase (PAL) labelling<\/li>\n<li>Biotin labelling<\/li>\n<li>Labelled with a fluorescent marker (Fluorescein FITC, Cyanine, Alexa fluor,&#8230;)<\/li>\n<\/ul>\n<p>The antibody couplings are executed according to robust protocols, carried out routinely by our teams.<\/p>\n<p>After modification of the antibody and quality control, the sample is shipped to the customer with a full report.[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88280&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_id=&#8221;plateforme-luminex-monoclonaux&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88470&#8243; back_position=&#8221;right center&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; radius=&#8221;std&#8221; shadow=&#8221;sm&#8221; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; back_size=&#8221;content&#8221;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-142237&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; el_id=&#8221;luminex-plateform&#8221;]LUMINEX platform[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;103545&#8243;]<strong>Partnership agreement with <a href=\"https:\/\/www.luminexcorp.com\/eu\/\" target=\"_blank\" rel=\"noopener\">Luminex Corporation<\/a><\/strong><\/p>\n<p><span style=\"font-weight: 400;\">RD-Biotech now offers<a href=\"https:\/\/rd-biotech.tetedecom.pro\/wp-content\/uploads\/2024\/01\/Luminex-platform-with-RD-Biotech-2023.png\" target=\"_blank\" rel=\"noopener\"> a range of custom services<\/a> with Luminex xMAP\u00ae technology!<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Antigen or antibody coupling with Luminex beads<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Analytical service: detection and quantification of analytes, mono or multiplex analysis using the Luminex 200 equipments\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Development of multiplexed assay<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">From providing labeled analytes to assay development, RD-Biotech can help you get to your Multiplexed assay\u2014faster.<\/span><\/p>\n<p><b>Advantages of Luminex technology<\/b><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Multiplex analysis of up to 100 analytes<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> High sensitivity<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Low sample volume<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Limited matrix interference<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Rapid analysis<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;192026&#8243; row_name=&#8221;Development of monoclonal antibodies&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;7&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;&#8221; overlay_alpha=&#8221;&#8221; shift_x=&#8221;&#8221; shift_y=&#8221;&#8221; shift_y_down=&#8221;&#8221; z_index=&#8221;&#8221; medium_width=&#8221;4&#8243; mobile_width=&#8221;&#8221; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Development of monoclonal antibodies&#8221; el_class=&#8221;icon_box_with_text&#8221;][\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;4&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_column_text]RD-Biotech offers support for your monoclonal antibody projects in mice, with the supply of validated clone cell banks.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text css_animation=&#8221;bottom-t-top&#8221;]<span style=\"font-weight: 400;\">Our technical and scientific expertise, combined with that of the mAbexpert group, allow us to offer:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The support for the expression of antigens (sequence analysis, preparation of peptides or recombinant proteins<br \/>\n<\/span><a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/recombinant-proteins\" rel=\"noopener\">Discover recombinant proteins<\/a><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">An extended screening platform: ELISA, Western Blotting, Cytometry, Octet\u2026<\/span><br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/analytical-services\" rel=\"noopener\"><span style=\"font-weight: 400;\">Discover analytical services<\/span><\/a><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The characterization of the monoclonal antibodies developed: specificity, affinity, bioactivity<br \/>\n<\/span><a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/analytical-services\" rel=\"noopener\"><span style=\"font-weight: 400;\">Discover analytical services<\/span><\/a><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The expertise and technological tools to secure your clone or to switch to recombinant antibodies (antibody sequencing, chimerization, humanization, etc.)<br \/>\n<\/span><a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/recombinant-antibodies\" rel=\"noopener\"><span style=\"font-weight: 400;\">Discover recombinant antibodies<\/span><\/a><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The generation of hybridomas in other species (rat, guinea pig)<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Phage Display technologies (mouse, rat, other species)<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;full-colored&#8221; border_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fwp-content%2Fuploads%2F2021%2F07%2FMonoclonal-antibody-development-service-hybridoma-scaled.jpg|target:_blank&#8221;]Discover the monoclonal antibody development service (hybridoma)[\/vc_button][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;500&#8243; css_animation=&#8221;bottom-t-top&#8221;]The different stages of an immunization program and hybridoma generation are described below, based on a <i>Go No Go<\/i> procedure.<\/p>\n<p><span style=\"font-weight: 400;\">Before starting a new project, RD-Biotech advises to perform a <\/span>preparatory meeting <span style=\"font-weight: 400;\">that will validate the different stages of the process: antigens, immunization routes, choice of host, mode of screening, differential screening, etc.<\/span>[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243;]Preparation of the immunogen[\/vc_custom_heading][vc_column_text]<b>Starting material provided by the customer:<\/b><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">The immunogen or group of immunogens: protein of interest, native or recombinant, peptide, peptide sequence in a computer file, &#8230;<\/span><\/li>\n<li><span style=\"font-weight: 400;\">The molecules necessary for the screening steps (antibodies, protein peptides, cell lysate, etc.)<\/span><\/li>\n<\/ul>\n<p><strong>RD-Biotech can also take on charge the preparation of immunogen(s)<\/strong><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Feasibility study: antigen design, sequence analysis, optimization,&#8230;<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Production of the immunogen, coupling to a carrier, &#8230;<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Frecombinant-proteins&#8221;]Discover the recombinant proteins[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88480&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88482&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243;]Hybridoma Generation[\/vc_custom_heading][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Immunization&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545162383366908816238337929571623833902835&#8243; slug=&#8221;immunization&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Immunization of 4 mice (standard package that can be adapted according to the project)<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Immunization of mice : footpad injection (intrapodal) (28 days), or intraperitoneal injection (49 days) <\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Number of injections and boosts adapted to the immunization protocol.<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The immune response is followed by ELISA titration or Western-blotting.<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The results are sent to the client.<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><em>Go No Go<\/em>: validation of the titration results by the customer<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Cell fusion&#8221; tab_id=&#8221;6dad8f3c-7309-81617960985185162376787651716238331504211623833317545162383366908816238337929571623833902835&#8243; slug=&#8221;cell-fusion&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"text-align: left;\"><span style=\"font-weight: 400;\">The fusion is carried out after selection of the mice in agreement with the client.<\/span><\/li>\n<li style=\"text-align: left;\"><span style=\"font-weight: 400;\">Maintenance of hybridomas on selective medium<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Screenings&#8221; tab_id=&#8221;1623833716180-2-816238337929571623833902835&#8243; slug=&#8221;screenings&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0Screening of all hybridoma supernatants by ELISA<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Other methods can be proposed: Dot-Blot, Western blotting, flow cytometry, Octet or other methods.<br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/analytical-services\">Discover analytical services<\/a><\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">After primary screening, the positive hybridomas are subcultured for secondary control<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Isotyping and saving of significantly positive hybridomas<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Possibility of sending positive supernatants to the client for additional tests and for the selection of the best performing hybridomas<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><i><span style=\"font-weight: 400;\">Go No GO<\/span><\/i><span style=\"font-weight: 400;\">: choice of candidates for cloning<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Cloning of selected positive hybridomas&#8221; tab_id=&#8221;1623833983125-3-0&#8243; slug=&#8221;cloning-of-selected-positive-hybridomas&#8221;][vc_column_text]<\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Cloning by two rounds of limiting dilution<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Screening of the supernatants after each series of cloning (ELISA or other methods of your choice)<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">We pay special attention in the early stages of cloning to avoid the loss of hybrids which may be of interest but unstable before the monoclonal stage.<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Isotyping of clones (<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/fastelisa-kits\/\">FastELISA<\/a> rdb-3255 Kit)\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\">If necessary, preparation of mini-productions and shipments to the customer for additional controls<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Master cell bank&#8221; tab_id=&#8221;1623834008567-4-9&#8243; slug=&#8221;master-cell-bank&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"text-align: left;\"><span style=\"font-weight: 400;\">Constitution of master banks for each selected clone with the freezing of 10 vials of 5 million cells<\/span><\/li>\n<li style=\"text-align: left;\"><span style=\"font-weight: 400;\">Additional vials are prepared for bank controls (freeze\/thaw viability and absence of mycoplasma contamination)<\/span><\/li>\n<li style=\"text-align: left;\"><span style=\"font-weight: 400;\">Safe storage of banks (to be specified)<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; row_name=&#8221;Monoclonal antobody sequencing&#8221; uncode_shortcode_id=&#8221;199235&#8243;][vc_column column_width_percent=&#8221;85&#8243; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Monoclonal antibody sequencing&#8221; el_class=&#8221;icon_box_with_text&#8221;][\/vc_icon][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; 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media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;115968&#8243; row_name=&#8221;Candidate characterization&#8221;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;7&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;&#8221; overlay_alpha=&#8221;&#8221; shift_x=&#8221;&#8221; shift_y=&#8221;&#8221; shift_y_down=&#8221;&#8221; z_index=&#8221;&#8221; medium_width=&#8221;4&#8243; mobile_width=&#8221;&#8221; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Candidate characterization&#8221; el_class=&#8221;icon_box_with_text&#8221;][\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;4&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_column_text]<span style=\"font-weight: 400;\">RD-Biotech offers different methods for the characterization of candidates: specificity, biological activity&#8230;<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> ELISA<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Western Blotting<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Flow cytometry<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Octet<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Bioassay platform (ADCC, CDC, etc.)<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fanalytical-services%2F&#8221;]Discover analytical services[\/vc_button][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; 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shift_x=&#8221;&#8221; shift_y=&#8221;&#8221; shift_y_down=&#8221;&#8221; z_index=&#8221;&#8221; medium_width=&#8221;4&#8243; mobile_width=&#8221;&#8221; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Clone rescue&#8221; el_class=&#8221;icon_box_with_text&#8221;][\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;4&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_column_text]Different strategies can be implemented, including the recloning of the clones of interest.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;120236&#8243; row_name=&#8221;Development and production of polyclonal antibodies&#8221;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;7&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;&#8221; position_horizontal=&#8221;left&#8221; 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