{"id":90476,"date":"2021-03-25T12:21:50","date_gmt":"2021-03-25T11:21:50","guid":{"rendered":"https:\/\/rd-biotech.tetedecom.pro\/anticorps-recombinants\/"},"modified":"2025-06-02T16:38:14","modified_gmt":"2025-06-02T14:38:14","slug":"recombinant-antibodies","status":"publish","type":"page","link":"https:\/\/rd-biotech.tetedecom.pro\/en\/recombinant-antibodies\/","title":{"rendered":"Recombinant antibodies"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row unlock_row=&#8221;&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shadow=&#8221;std&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;-4&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;2&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;107504&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;6&#8243; mobile_width=&#8221;7&#8243; width=&#8221;5\/6&#8243; uncode_shortcode_id=&#8221;115501&#8243;][vc_custom_heading heading_semantic=&#8221;p&#8221; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;205989&#8243; text_color_type=&#8221;uncode-solid&#8221; text_color_solid=&#8221;#ffcc00&#8243;]<span style=\"font-weight: 400;\">RD-Biotech offers a complete range of custom services with its Antibody Engineering platform.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">By contacting RD-Biotech you will:<\/span><\/p>\n<ul>\n<li>save time while devoting yourself to your major areas of high added value research,<\/li>\n<li>benefit from our scientific and technical expertise in immunology and antibody engineering.<\/li>\n<\/ul>\n<p>[\/vc_custom_heading][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;2&#8243; mobile_width=&#8221;7&#8243; width=&#8221;1\/6&#8243; uncode_shortcode_id=&#8221;747647&#8243;][vc_single_image media=&#8221;99628&#8243; media_width_percent=&#8221;80&#8243; alignment=&#8221;center&#8221; uncode_shortcode_id=&#8221;421845&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;4&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;133869&#8243;]An integrated platform for the development of your therapeutic antibodies: from target to candidate[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_speed=&#8221;1000&#8243; width=&#8221;1\/1&#8243;]\n\t\t<div id=\"tdc_summary_876983\" class=\"tdc_summary text-lead wpb_content_element \">\n\n\t\t\t<div class=\"tdc_summary_title\">\n\t\t\t\t<a href=\"#\" class=\"summaryAnchor\">Summary<i class=\"fa fa-chevron-down close\" aria-hidden=\"true\"><\/i><\/a>\n\t\t\t<\/div>\n\n\t\t<\/div>\n\t\t[\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;&#8221; overlay_alpha=&#8221;&#8221; shift_x=&#8221;&#8221; shift_y=&#8221;&#8221; shift_y_down=&#8221;&#8221; z_index=&#8221;&#8221; medium_width=&#8221;&#8221; mobile_width=&#8221;&#8221; width=&#8221;4\/12&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Bioproduction&#8221; el_id=&#8221;bioproduction&#8221; el_class=&#8221;icon_box_with_text&#8221;][\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;8\/12&#8243;][vc_column_text uncode_shortcode_id=&#8221;141837&#8243;]RD-Biotech is a service company recognized for its know-how and experience in the field of antibodies. In particular, the company offers multi-technology services for the development of antibodies for R&amp;D, diagnostic and therapeutic purposes.<\/p>\n<p>Production of your candidates according to an adapted and personalized strategy according to the biological tools and the available data:[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_empty_space empty_h=&#8221;2&#8243;][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;THE CUSTOMER CAN PROVIDE&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545162383366908816238337929571623834218913&#8243; slug=&#8221;le-client-peut-fournir&#8221;][vc_column_text el_class=&#8221;v-tabs-larger&#8221; uncode_shortcode_id=&#8221;157362&#8243;]<\/p>\n<ul>\n<li class=\"panel-title active\">The target (proteins, peptide, protein sequence, bibliographic data)<\/li>\n<li class=\"panel-title active\">The murine monoclonal antibody secreting clone<\/li>\n<li class=\"panel-title active\">Nucleotide sequences of interest (VH and VL sequences)<\/li>\n<li class=\"panel-title active\">Expression vectors ready-to-use for transfection<\/li>\n<li class=\"panel-title active\">The cell lines<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;RD-BIOTECH EXPERTISE&#8221; tab_id=&#8221;6dad8f3c-7309-81617960985185162376787651716238331504211623833317545162383366908816238337929571623834218913&#8243; slug=&#8221;expertise-rd-biotech&#8221;][vc_column_text uncode_shortcode_id=&#8221;284811&#8243;]<strong>Depending on the progress level of your project, RD-Biotech has the expertise and the technological platforms to:<\/strong><\/p>\n<ul>\n<li>Express your target (vector construction and antigen expression)<\/li>\n<li>Develop monoclonal antibodies (Hybridomas and phage display)<\/li>\n<li>Sequence monoclonal antibodies<\/li>\n<li>Develop and produce your recombinant antibodies<\/li>\n<li>Recombinant mice or other species, different isotypes possible<\/li>\n<li>Chimerization of your candidate<\/li>\n<li>Humanization of your candidate<\/li>\n<li>Genetic modifications: Reformatting with another species, change of isotypes, antibody fragments (Fab, scFv, nanobodies), bispecific format, \u2026<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;RD-BIOTECH QUALITY&#8221; tab_id=&#8221;1623833716180-2-816238337929571623834218913&#8243; slug=&#8221;qualite-rd-biotech&#8221;][vc_column_text uncode_shortcode_id=&#8221;175010&#8243;]<\/p>\n<ul>\n<li aria-level=\"1\">Production system optimized in mammalian cells: CHO cells, HEK cells (other cells \u2013\u00a0<a href=\"https:\/\/web.archive.org\/web\/20230128055022\/https:\/\/rd-biotech.tetedecom.pro\/en\/contact\/\">contact us<\/a>)<\/li>\n<li aria-level=\"1\">Transfection from vectors optimized for mammalian cells<\/li>\n<li aria-level=\"1\">R&amp;D \u201cendofree\u201d quality or High Quality Grade<\/li>\n<li aria-level=\"1\">Reproducibility: in vitro procedure, chemically defined medium, for excellent inter-batch reproducibility<\/li>\n<li aria-level=\"1\">Purity: serum free mammalian cell expression system eliminating the risk of contamination by serum components, and providing a level of purity greater than 95%.<\/li>\n<li aria-level=\"1\">Capacity: pilot batch production or large-scale production (up to several hundred mgs)<\/li>\n<li aria-level=\"1\">Speed: high-performance expression system allowing the supply of batches of recombinant antibodies in a very short time: Supply of a batch of 100 mg of recombinant antibodies purified and checked in less than 3 weeks.<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;147252&#8243; row_name=&#8221;Antibody production by transient transfection&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; el_id=&#8221;production-d-anticorps-par-transfection-transitoire&#8221; uncode_shortcode_id=&#8221;996910&#8243;]Antibody production by transient transfection[\/vc_custom_heading][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;500&#8243; text_italic=&#8221;yes&#8221; uncode_shortcode_id=&#8221;557634&#8243;]CHO, HEK293 or other cells[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;835520&#8243;]The standard procedure includes the following steps (certain steps may be carried out by the customer, to be specified)<\/p>\n<ul>\n<li aria-level=\"1\"><a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/molecular-biology\/#antibody-sequencing-molecular-biology\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">Optional step: monoclonal antibody sequencing, heavy and light chains<\/span><\/a><\/li>\n<li aria-level=\"1\">1st step : synthesis of variable fragments VH and VL<\/li>\n<li aria-level=\"1\">2nd step : subcloning the fragments into the expression vector. Quality controls.<\/li>\n<li aria-level=\"1\">3rd step : antibody expression pilot by transient transfection.<\/li>\n<li aria-level=\"1\">4th step : production monitoring by ELISA (<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/fastelisa-kits\/\" rel=\"noopener\">Discover FastELISA<\/a>)<\/li>\n<li aria-level=\"1\">5th step :purification\u00a0of the pilot by affinity chromatography (Protein A support)<\/li>\n<li aria-level=\"1\">6th step : Quality controls<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/analytical-services\" rel=\"noopener\">(analytical services)<\/a>\n<ul>\n<li aria-level=\"1\">Purity control by SDS-PAGE gel electrophoresis (reducing and non-reducing conditions)<\/li>\n<li aria-level=\"1\">Determination of total proteins by absorbance measurement (spectrophotometer)<\/li>\n<li aria-level=\"1\">Other controls possible,\u00a0<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/contact\/\">consult us<\/a><\/li>\n<\/ul>\n<\/li>\n<li aria-level=\"1\">7th step\u00a0<b>:\u00a0<\/b>Packaging and shipment of the mAb, accompanied by report.<\/li>\n<\/ul>\n<p><em>Deadlines :<br \/>\nStep 2: 2 to 3 weeks<br \/>\nSteps 3 to 5: approximately 3 weeks<\/em><\/p>\n<p>For a Fastrack service: contact us![\/vc_column_text][vc_single_image media=&#8221;91306&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; uncode_shortcode_id=&#8221;173385&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;154054&#8243;][vc_single_image media=&#8221;99858&#8243; media_width_percent=&#8221;100&#8243; media_ratio=&#8221;three-two&#8221; uncode_shortcode_id=&#8221;793698&#8243;][vc_single_image media=&#8221;99856&#8243; media_width_percent=&#8221;100&#8243; media_ratio=&#8221;three-two&#8221; uncode_shortcode_id=&#8221;189211&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;99860&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;124890&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;8\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h3&#8243; uncode_shortcode_id=&#8221;304239&#8243;]Go No Go: Large Scale production and purification (up to gram scale) according to the validated procedure.[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;142865&#8243;]Optionally, the antibodies produced in vitro can be purified under \u201cEndofree\u201d conditions, which requires the use of non-pyrogenic buffers and containers.<\/p>\n<p>After the affinity chromatography step, the purity of the antibody can be improved by different methods:<\/p>\n<ul>\n<li>by a\u00a0<strong>preparative SEC<\/strong>\u00a0step which will eliminate the aggregates;<\/li>\n<li>by an<strong>\u00a0ion exchange chromatography<\/strong>\u00a0step, which also removes any endotoxins that may be present. The monoclonal antibodies produced in vitro by RD-Biotech under \u201cEndofree\u201d conditions have an\u00a0<strong>endotoxin level of less than 1 EU \/ mg.<\/strong><\/li>\n<\/ul>\n<p>Additional quality controls can be added to the basic controls, depending on the specifications of the ordering customer and the applications:[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Additional quality controls&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517162383315042116238333175451623833669088162383379295716255805822591625582725134&#8243; slug=&#8221;controles-qualite-supplementaires&#8221;][vc_column_text uncode_shortcode_id=&#8221;573515&#8243;]<\/p>\n<ul>\n<li>Mycoplasma test<\/li>\n<li>LAL test to determine the level of bacterial endotoxins. The LAL (Limulus Amebocyte Lysate) test is performed using a regulatory kit according to the supplier\u2019s protocol.<\/li>\n<li>Bacterial and fungal contamination test<\/li>\n<li>Control by analytical SEC<\/li>\n<li>Desalting of antibodies<\/li>\n<li>Control of specificity, biological activity \u2026 (ELISA, Western Blotting, Octet, Cytometry, custom bioassays \u2026)<\/li>\n<li>Protein A residue determination<\/li>\n<li>Determination of bovine Ig contaminants (RD-Biotech FastELISA Kit)<br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/fastelisa-kits\/\" rel=\"noopener\">Discover FastELISA<\/a><\/li>\n<li>As part of the High Quality Grade process, specific controls are applied<br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/contact\/\" rel=\"noopener\">Contact us<\/a><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fanalytical-services%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;154044&#8243;]Discover analytical services[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;244147&#8243; row_name=&#8221;Production by stable transfection&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shadow=&#8221;std&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;186770&#8243;]Production by stable transfection[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;203927&#8243;]Production of recombinant antibodies from a cell line or a stable pool of cells, supplied by the ordering customer or developed by RD-Biotech[\/vc_column_text][vc_single_image media=&#8221;91308&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; uncode_shortcode_id=&#8221;138794&#8243;][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fcell-culture%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;409859&#8243;]Discover cell culture[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;99862&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;214591&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;712562&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;&#8221; overlay_alpha=&#8221;&#8221; shift_x=&#8221;&#8221; shift_y=&#8221;&#8221; shift_y_down=&#8221;&#8221; z_index=&#8221;&#8221; medium_width=&#8221;&#8221; mobile_width=&#8221;&#8221; css_animation=&#8221;left-t-right&#8221; width=&#8221;5\/12&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Custom contract services&#8221; el_class=&#8221;icon_box_with_text&#8221; uncode_shortcode_id=&#8221;173603&#8243;][\/vc_icon][vc_button button_color=&#8221;color-109923&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fwp-content%2Fuploads%2F2023%2F06%2FRD-Biotech_-Antibody-recombinant-platform.jpg|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;142441&#8243;]Find out more[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;7\/12&#8243;][vc_column_text uncode_shortcode_id=&#8221;119066&#8243;]Recombinant antibodies have undeniable advantages because they are produced according to an animal free process, in a chemically and biologically defined environment, unlike hybridomas.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_column_text uncode_shortcode_id=&#8221;105248&#8243;]RD-Biotech puts its expertise and know-how in molecular &amp; cell engineering at the service of its customers to modify recombinant antibodies and generate variants according to your specifications.<\/p>\n<ul>\n<li>Success rate&gt; 98%<\/li>\n<li>Scale-up of up to several hundred mg<\/li>\n<li>Up to 50 variants<\/li>\n<li>Royalty-free<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_column_text uncode_shortcode_id=&#8221;160269&#8243;]<strong>Benefits :<\/strong><\/p>\n<ul>\n<li>Purity: can reach very high levels (&gt; 98%) by expression in mammalian cells and without serum, eliminating the risk of contamination by serum components<\/li>\n<li>Reduction of production times<\/li>\n<li>Process reproducibility<\/li>\n<li>Antibody engineering<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;692375&#8243; row_name=&#8221;Antibody sequencing&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;5\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;347108&#8243;]Antibody sequencing[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;913990&#8243;]RD-Biotech can carry out the heavy and light chain sequencing of the monoclonal antibody candidate[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fmolecular-biology%2F%23sequencage-anticorps-monoclonaux-bio-mol|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;138146&#8243;]Discover molecular biology antibody sequencing[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;99864&#8243; back_position=&#8221;right center&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;7\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;193959&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;623040&#8243; row_name=&#8221;Antibody reformatting&#8221;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shadow=&#8221;std&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][vc_single_image media=&#8221;91310&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;75&#8243; alignment=&#8221;center&#8221; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; uncode_shortcode_id=&#8221;764269&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-109607&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;979155&#8243;]Antibody reformatting[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;286856&#8243;]Reformatting service for your antibodies: scFv, Fab fragments, bispecific, change of isotypes or sub-isotypes \u2026[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fcontact%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;168228&#8243;]Contact us[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;130716&#8243; row_name=&#8221;Chimerization&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-142237&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;634729&#8243;]Chimerization[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;615374&#8243;]Chimeric antibodies are formed by the association of the variable regions of a \u201cparental\u201d or \u201cdonor\u201d antibody with the constant regions of an \u201cacceptor\u201d antibody (of a different species). The combination of murine variable domains and human constant domains is the most common, but other combinations are also possible.<\/p>\n<p>Chimerization is a simple and straightforward process for retaining the affinity and specificity of the parental antibody.[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;THE STANDARD PROCEDURE CONSISTS OF THE FOLLOWING STEPS&#8221; tab_id=&#8221;d21d5937-3bb0-2161796098518516237678765171623833150421162383331754516238336690881623833792957162558058225916255827251341625582926978&#8243; slug=&#8221;etapes-procedure-chimerisation&#8221;][vc_column_text uncode_shortcode_id=&#8221;520729&#8243;]<\/p>\n<ul>\n<li aria-level=\"1\">Development of the parental monoclonal antibody (if requested)<\/li>\n<li aria-level=\"1\">Sequencing of the VH and VL variable fragments of the parental antibody (if requested)<\/li>\n<li aria-level=\"1\">Construction of expression vectors: codon optimization, selection of constant domains (species and isotype), cloning of VH and VL variable domains. Quality controls.<br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/plasmid-dna\/\" rel=\"noopener\">Discover molecular biology<\/a><\/li>\n<li aria-level=\"1\">Co-transfection and production of chimeric antibodies by transient transfection in CHO cells<br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/recombinant-antibodies\/#production\" rel=\"noopener\">Discover recombinant antibodies production<\/a><\/li>\n<li aria-level=\"1\">Productivity evaluation by ELISA (FastELISA kits)<br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/fastelisa-kits\/\" rel=\"noopener\">Discover FastELISA<\/a><\/li>\n<li aria-level=\"1\">Purification by affinity chromatography (protein resin A, G, L, etc.)<\/li>\n<li aria-level=\"1\">Quality controls:<br \/>\n<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/analytical-services\" rel=\"noopener\">(analytical services)<\/a><\/p>\n<ul>\n<li aria-level=\"1\">Purity control by SDS-PAGE gel electrophoresis (reducing and non-reducing conditions)<\/li>\n<li aria-level=\"1\">Determination of total proteins by absorbance measurement (spectrophotometer)<\/li>\n<li aria-level=\"1\">Additional controls offered (depending on the project)\n<ul>\n<li aria-level=\"1\">SEC HPLC<\/li>\n<li aria-level=\"1\">Determination of affinity<\/li>\n<li aria-level=\"1\">Functional tests<\/li>\n<li aria-level=\"1\">Other tests available<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fcontact%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;120871&#8243;]Contact us[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88280&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;107798&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;169919&#8243; row_name=&#8221;Humanization&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88246&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-gyho&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;8\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;661299&#8243;]Humanization[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;633148&#8243;]Humanized antibodies constitute the majority of therapeutic antibodies approved on the market.<\/p>\n<p>The humanization of a parental antibody (eg mouse) is carried out according to a modernized version of \u201cCDR-grafting\u201d according to the approach invented by Winter et al. (\u201cMedical Research Council\u201d \u2013 Cambridge \u2013 UK). The principle of this method is to reformat a human antibody (germline sequences) so that it contains the CDRs (Complementary Determinant Regions) of the parental antibody in order to reduce its immunogenicity in humans. Humanization by \u201cCDR-grafting\u201d requires that mouse antibody residues involved in antigen recognition be preserved in the humanized version of the antibody.<\/p>\n<p>Following the humanization step, sequence optimization may be offered with optional additional analyses by molecular modelling:<\/p>\n<p><b>The objective being:<\/b><\/p>\n<ul>\n<li>An increase in manufacturability (reduction of heterogeneity)<\/li>\n<li>A possible increase in affinity and biological activity (IC50)<\/li>\n<\/ul>\n<p><strong>The optimized procedure proposed by RD-Biotech includes the steps described below:<\/strong>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;STEP 1: IN SILICO HUMANIZATION OF THE RECOMBINANT ANTIBODY&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545162383366908816238337929571623834301776&#8243; slug=&#8221;etape-1-humanisation&#8221;][vc_column_text el_class=&#8221;v-tabs-larger&#8221; uncode_shortcode_id=&#8221;233971&#8243;]<strong>Humanization by CDR-grafting:<\/strong><\/p>\n<ul>\n<li>Identification of framework regions (FRs) and CDRs of the parental antibody.<\/li>\n<li>Search and alignment of parental antibody sequences with the most homologous human germ sequences<\/li>\n<li>Identification of critical positions.<\/li>\n<li>Selection of a germline sequence for each chain (VH and VL) based on sequence analysis and identification of critical positions<\/li>\n<li>Assessment of the suitability of the substitution at each diverging position between the murine and human sequences based on structural analysis.<\/li>\n<li>CDR-grafting by analysing sequence differences between parental sequences and acceptor sequences of the antibody.<\/li>\n<\/ul>\n<p><strong>Sequence optimization:<\/strong><\/p>\n<ul>\n<li>Improving the manufacturability of the antibody and optimizing its affinity and activity.<\/li>\n<li>Provision of an interim report including strategy, aa sequences of VL and VH of humanized variants to be synthesized and tested as well as suggestions for sequence optimization.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fmolecular-biology%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;150878&#8243;]Discover molecular biology[\/vc_button][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;STEP 2: HUMANIZED RECOMBINANT ANTIBODY&#8221; tab_id=&#8221;6dad8f3c-7309-81617960985185162376787651716238331504211623833317545162383366908816238337929571623834301776&#8243; slug=&#8221;etape-2-humanisation&#8221;][vc_column_text uncode_shortcode_id=&#8221;178151&#8243;]For each construction, the stages of construction of the expression vectors, co-transfection, production and purification are carried out, according to the same procedures as those described in the previous chapter \u201cproduction by transient transfection\u201d<\/p>\n<p>Quality controls include:<\/p>\n<ul>\n<li aria-level=\"1\">Purity control by SDS-PAGE gel electrophoresis (reducing and non-reducing conditions)<\/li>\n<li aria-level=\"1\">Determination of total proteins by absorbance measurement (spectrophotometer)<\/li>\n<li aria-level=\"1\">Additional controls offered (depending on the project)\n<ul>\n<li aria-level=\"1\">SEC HPLC<\/li>\n<li aria-level=\"1\">Determination of affinity<\/li>\n<li aria-level=\"1\">Functional tests (bioassay platform)<\/li>\n<li aria-level=\"1\">Other tests available,\u00a0<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/contact\/\">contact us<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>At the end of this step, a report is issued, including, for each candidate, the strategy followed, the results obtained and certificates of analysis.[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;STEP 3: SECOND CYCLE OF HUMANIZATION AND OPTIMIZATION OF SEQUENCES&#8221; tab_id=&#8221;1623833716180-2-816238337929571623834301776&#8243; slug=&#8221;etape-3-humanisation&#8221;][vc_column_text uncode_shortcode_id=&#8221;104494&#8243;]From an identified \u201clead candidate\u201d, a second humanization cycle can be proposed in order to substitute any murine residues with their human counterparts in the \u201cnon-CDR\u201d regions and thus increase the human % in VH and VL<\/p>\n<ul>\n<li>Sequence optimization to eliminate potential PTMs present in CDRs<\/li>\n<li>Complementary analysis by modelling<\/li>\n<\/ul>\n<p>These different optimization approaches can be carried out in parallel.[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fmolecular-biology%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;744502&#8243;]Discover molecular biology[\/vc_button][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;126623&#8243; row_name=&#8221;Characterization and validation of candidates&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;169712&#8243;]Characterization and validation of candidates[\/vc_custom_heading][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;500&#8243; text_italic=&#8221;yes&#8221; uncode_shortcode_id=&#8221;123572&#8243;]specificity, affinity, bioactivity, &#8230;[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;199230&#8243;]RD-Biotech\u2019s analytical platform offers a wide range of services:<\/p>\n<ul>\n<li>Concentration, Purity<\/li>\n<li>Determination of endotoxins levels<\/li>\n<li>Determination of the aggregate rate<\/li>\n<li>Determination of the residual rate of contaminants: proteins A,\u2026.<\/li>\n<li>Sequencing of the variable parts VH and VL<\/li>\n<li>Analysis of the specificity of recombinant antibodies: ELISA, Western Blot, etc.<\/li>\n<li>Analysis of the affinity of recombinant antibodies: determination of Kd, Kon, Koff using Octet technology<\/li>\n<li>Stability analysis: DSC<\/li>\n<li>Analysis of the biological activity of antibodies: wide range of bioassays (ADCC, ADCP, CDC, \u2026 consult us) and custom development service<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fanalytical-services%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;155568&#8243;]Discover analytical services[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88491&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;854910&#8243; row_name=&#8221;Phage display&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;99866&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;120094&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;8\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;515778&#8243;]Phage display[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;168383&#8243;]Thanks to the know-how of the mAbexperts team, RD-Biotech offers the development of recombinant antibodies using Phage Display technology:<\/p>\n<p><strong>Phage display in mice:<\/strong><\/p>\n<ul>\n<li>Excellent alternative to hybridoma technology<\/li>\n<li>Fast process (3 to 5 months)<\/li>\n<li>Provision of naive or immune banks (fab, scFv)<\/li>\n<\/ul>\n<p><strong>Phage display in llamas:<\/strong><br \/>\nDevelopment of your VHH by immunization of llamas and using phage display technology.[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fcontact%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;113279&#8243;]Contact us[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;5&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shadow=&#8221;std&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_custom_heading text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; uncode_shortcode_id=&#8221;681367&#8243;]Other services[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;108162&#8243;]Our sales team as well as our scientific and technical experts are at your disposal to offer you the service adapted to your request![\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:https%3A%2F%2Frd-biotech.tetedecom.pro%2Fen%2Fcontact%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;365422&#8243;]Contact us[\/vc_button][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row unlock_row=&#8221;&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shadow=&#8221;std&#8221; shift_x=&#8221;0&#8243; shift_y=&#8221;-4&#8243; [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":99875,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-90476","page","type-page","status-publish","has-post-thumbnail","hentry","page_category-services-en"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>RD-Biotech, recombinant antibody development and production services<\/title>\n<meta name=\"description\" content=\"Recombinant antibody expression and cloning services, humanization, reformatting and antibody characterization services\" \/>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RD-Biotech, recombinant antibody development and production services\" \/>\n<meta property=\"og:description\" content=\"Recombinant antibody expression and cloning services, humanization, reformatting and antibody characterization services\" \/>\n<meta property=\"og:url\" content=\"https:\/\/rd-biotech.tetedecom.pro\/en\/recombinant-antibodies\/\" \/>\n<meta property=\"og:site_name\" content=\"RD-BIOTECH\" \/>\n<meta property=\"article:modified_time\" content=\"2025-06-02T14:38:14+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/rd-biotech.tetedecom.pro\/wp-content\/uploads\/2023\/06\/RD-B_2023-AcRec2.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"800\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta 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