Plasmid construction
Every year, RD-Biotech designs and produces more than 1,000 plasmids for over 500 clients (including academic researchers, industrial R&D teams, and biotech companies in clinical development).
Expression vectors, reporter plasmids, multicistronic constructs, and CRISPR tools: the wide variety of requests we receive reflects the breadth of fields involved, from fundamental cell biology to gene therapy applications.
This is why our approach always begins by listening: understanding your context, current stage, and objectives. Whether you have a finalized design or are still looking for the best strategy, our experts adapt to your needs and recommend the most suitable solution.
Beyond vector construction, RD-Biotech can support you through to recombinant protein expression—whether you wish to outsource the entire workflow or benefit from in-house functional validation.
Custom Plasmids
The starting point for plasmid construction is rarely the same from one project to another. That is why we have developed an approach with no prerequisites: you provide whatever material or information you have, and we take care of the rest.
Starting material may take many forms: a coding or regulatory nucleic acid sequence, a protein sequence, a GenBank or UniProt identifier, or simply a bibliographic reference. In the latter case, our experts identify and retrieve the sequence of interest on your behalf.
Several vector options are available: we can select the backbone best suited to your expression system from our in-house collection, or work with your own acceptor vector under strict confidentiality. You may also provide us with a plasmid sequence that you do not physically possess, and we will reconstruct it.
The diversity of the constructs we produce—including large inserts, high-GC sequences, repeated elements, and toxic fragments—demonstrates our command of a broad range of cloning strategies, selected and adapted to the specific requirements of each project.
What you provide ↓
Custom plasmid construction ↓
Applications and intended uses ↓
Deliverables
All vectors cloned by RD-Biotech come with a 100% sequence-fidelity guarantee. The quality-control checks applied to custom vector cloning are listed below.
Agarose gel electrophoresis
- Undigested profile
- Restriction digest profile
Polyacrylamide gel electrophoresis
- Poly(A) digestion profile, where applicable
- Other quality-control tests available on request
- Sanger sequencing of the insert
- Complete plasmid sequencing using Oxford Nanopore technology
- Complete plasmid sequencing by Sanger, on request
Plasmids for antibody and recombinant protein
The performance of a recombinant protein or antibody depends on the quality of the expression vector used. RD-Biotech supports you from the earliest stages of your project by designing plasmids tailored to your biological constraints, expression objectives, and end applications.
Whether you are working with antibodies in any format or with complex recombinant proteins, we optimize every component of the plasmid (including the sequence, promoters, tags, and secretion signals) to maximize product yield, solubility, and functionality.
Antibodies
All formats · All species · All isotypes
What you provide
Recombinant proteins
All protein types · All expression systems
What you provide
Tow operating models to meet your requirements
RD-Biotech adapts to your intellectual property requirements. For performance-driven projects, we use licensed technology that delivers transient antibody-expression yields of up to 1 g/L; in this model, the plasmid material remains the property of RD-Biotech.
For projects requiring technology transfer, we work with royalty-free vectors that are made fully available to the client upon project completion.
Plasmids for mRNA production by in vitro transcription
The quality of mRNA produced by in vitro transcription depends first and foremost on the quality of its plasmid template. The transcription promoter, 5′ and 3′ untranslated regions (UTRs), open reading frame (ORF), poly(A) tail, and linearization site all directly influence transcription yield, mRNA stability, and translation efficiency.
Plasmids for viral vector production and gene therapy
Viral vector production relies on a multiple-plasmid system whose quality and design directly determine the performance of the final vector. RD-Biotech has expertise in constructing all of these components—helper, packaging, and transfer plasmids—for the main systems used in gene therapy, primarily AAV and lentiviral vectors, with other systems available on request.
The transfer plasmid lies at the heart of our molecular biology work. It carries the transgene of interest and its associated regulatory elements—including the promoter, ITRs, packaging signals, and insulator elements. Its design can be fully customized according to your therapeutic target, AAV serotype, cell tropism, or insert-capacity constraints.
Plasmids for cell line development
Plasmid development services for generating your cell lines.
We support you in selecting key elements (including promoters, expression cassettes, and selection markers) to ensure stability, efficient expression, and lasting cell performance.
We design custom plasmids that are precisely tailored to your experimental objectives and applications.
