Recombinant protein biomanufacturing
Accelerate your bioproduction projects with comprehensive support, from in silico analysis through to the large-scale production of recombinant proteins.
Our customized solutions provide optimized yields, high quality and reliable processes tailored to research, preclinical and clinical applications.
In silico feasibility assessment and system selection
Before beginning recombinant protein production, we perform an in-depth in silico feasibility study covering:
- Sequence analysis
- Structure prediction
- Assessment of expression and stability risks
This scientific assessment enables us to select the optimal recombinant protein expression system (CHO, HEK, E. coli, yeast or cell-free) according to your target protein and its intended application, whether for research, diagnostics or therapeutic use.
The result: an informed decision, minimized risks and considerable time savings.
Expression plasmid construction and optimization
We provide optimized gene synthesis and design and construct your expression plasmid, incorporating codon optimization and regulatory elements suited to the selected system. The resulting plasmid is validated through quality-control testing.
Pilot study and small-scale production
Small-scale production enables us to validate expression, optimize conditions and characterize your protein in terms of purity, identity and biological activity. You receive a detailed report containing clear recommendations.
The pilot study is a decisive stage. It includes:
- Small-volume expression, from tens of millilitres in culture flasks
- Recombinant protein purification
- Comprehensive characterization using SDS-PAGE, Western blotting, protein quantification and activity testing
- Parallel assessment of multiple conditions to identify the optimal parameters
At the end of the proof-of-concept study, you receive a detailed report and a clear go/no-go recommendation before investing in larger-scale production.
Large scale up production & scaling-up
We provide controlled scale-up and large-scale batch production (up to several grams) using optimized processes, high-purity purification and comprehensive characterization. The resulting proteins are ready for preclinical or clinical applications.
From pilot production to research-scale batches (up to several hundred milligrams or litres of culture supernatant) we ensure controlled and reproducible scale-up.
Our services include:
- Recombinant protein purification by affinity chromatography or another suitable method
- Tag removal when required
- Concentration
- Aliquoting
- Comprehensive batch characterization
Every batch is supplied with a complete certificate of analysis and is ready for applications in immunology, recombinant protein vaccines or therapeutic development.
