Molecular Biology and Plasmid DNA: From R&D to GMP
From plasmid engineering to GMP manufacturing, accelerate your innovations in gene therapy, cell therapy, mRNA, and biotechnology.
Molecular biology and plasmid engineering for research and clinical phases: R&D, HQ, and GMP grades
+99%
of projects successfully delivered in accordance with client specifications
<3weeks
to receive your ready-to-use plasmid
100%
of personalized solutions tailored to your specific needs
Plasmids for mRNA production by in vitro transcription
We provide a high-quality template incorporating a promoter, UTRs, and a poly(A) tail of guaranteed length, supporting excellent in vitro transcription and translation performance.
+99%
of projects successfully delivered in accordance with client specifications
<3weeks
to receive your ready-to-use plasmid
100%
of personalized solutions tailored to your specific needs
GMP-grade plasmid biomanufacturing
Biomanufacturing in accordance with GMP standards for clinical applications and regulatory requirements, with comprehensive monitoring, quality control, and documentation at every stage.
+99%
of projects successfully delivered in accordance with client specifications
<3weeks
to receive your ready-to-use plasmid
100%
of personalized solutions tailored to your specific needs
+99%
of projects successfully delivered in accordance with client specifications
<3weeks
to receive your ready-to-use plasmid
100%
of personalized solutions tailored to your specific needs
The assurance of quality made in France
French scientific excellence supporting your innovations through quality, responsiveness, confidentiality, and protection of your intellectual property.
Why choose high-quality plasmid DNA?
Plasmid DNA is the starting point for many innovative biotherapies. Its quality, sequence fidelity, and regulatory compliance are essential to ensuring the reproducibility, safety, and clinical acceptance of your products.
Benefit from expert, customized support for your molecular biology and plasmid production projects
«From R&D through therapeutic development, leverage our scientific and technical expertise in molecular biology and plasmid DNA biomanufacturing. Optimize your time while focusing on your highest-value research priorities.»
Jonathan Mayali
Meet our molecular biology experts
Anthony
Head of Plasmid Vector Construction
Valentin
Head of HQ-Grade Plasmid Production
Justine
Head of R&D-Grade Plasmid Production
We meet our clients’ needs with advanced technical solutions while optimizing budgets.
Our molecular biology expertise: a practical example
Objective: To construct, characterize, and manufacture an HQ-grade plasmid intended for our client’s mRNA production.
- Construct design
- Cloning
- Supercoiled DNA and poly(A) stability studies
- R&D-grade production
- In vitro transcription to validate mRNA performance
- HQ cell bank
- HQ-grade production
- Quality control
FAQ
Do you have questions about our plasmid DNA services? Here is what you need to know to get your project off to a good start.
We offer a broad range of services:
- Plasmid vector construction, cloning, and mutagenesis
- Plasmid biomanufacturing at different scales and in R&D, HQ, and GMP grades
- Nucleic acid amplification, including PCR, RT-PCR, and qPCR
- Cell line validation and genotyping
- DNA and RNA preparation and purification
- Research Grade: Ideal for R&D and preclinical studies.
- High Quality Grade: Designed for advanced preclinical studies, toxicology studies, and certain early-stage clinical trials, including Phases I and II. Manufactured according to enhanced quality standards using a GMP-like approach.
- GMP Grade: Fully compliant with regulatory requirements for late-stage clinical trials, including Phase III, and commercial manufacturing.
We help you select the grade best suited to your stage of development.
Yes. This is one of our key strengths.
At RD-Biotech, we encourage cross-functional projects drawing on several areas of expertise, including hybridoma production, antibody sequencing, vector construction, amplification, recombinant expression, plasmid biomanufacturing, and cell line development and validation.
We ensure seamless coordination between our different platforms. Whether your project requires a fully integrated approach or simply the transfer of material between departments, we provide centralized and responsive project management.
We accept a wide variety of starting materials, including synthetic DNA, shuttle vectors, plasmid suspensions, transformed bacteria, and glycerol stocks.
We recommend providing 1–10 µg of starting plasmid. This is the ideal amount, although we can also work with smaller quantitie
Depending on the production scale and requested quality grade, bacterial cultures are grown in shake flasks or fermenters.
Purification is performed using commercial kits or an RD-Biotech-developed downstream-processing method.
Yes. You can provide your own protocols for us to implement directly through technology transfer, or we can optimize them together.
Flexibility is central to our approach.
Quality-control testing depends on the project and required quality grade. Concentration, the 260/280 nm ratio, and restriction-enzyme analysis are performed systematically.
Chaque prestation est accompagnée d’un rapport clair et complet, incluant selon le cas les conditions expérimentales, les résultats, leur interprétation et les contrôles qualité réalisés.
Additional testing can be provided upon request, including:
- LAL testing
- Insert or complete plasmid sequencing
- Detection of host-cell genomic DNA or RNA
- Bioburden testing
- pH measurement
Each service includes a clear and comprehensive report covering, as applicable, the experimental conditions, results, interpretation, and quality controls performed.
We retain an aliquot of every plasmid constructed or produced for you for at least 18 months.
This allows us to reproduce your plasmid quickly upon receipt of your purchase order. Contact us to confirm availability, and we will promptly let you know whether it can be returned to production immediately.
RD-Biotech is ISO 9001:2015 certified. We work according to good laboratory practices and can operate in a GMP environment for plasmid production intended for clinical use.
Yes. We have extensive expertise in large plasmids, unstable sequences, AAV ITRs, mRNA poly(A) tails, and other challenging constructs, covering both plasmid construction and production.
Our team can optimize the bacterial strain, culture medium, and production conditions to maximize yield and quality.
Simply send your request to the sales contact listed on the relevant service page or contact us by email.
The more information you provide about your project, the faster we can prepare an accurate, tailored proposal.
Discover our other services
RD-Biotech has been a biotechnology CRO/CDMO since 2002. We provide customized molecular biology, immunology, and cell-engineering solutions for R&D and preclinical and clinical development, combining expertise, responsiveness, and confidentiality.
Would you like to clarify your expectations?