Molecular biology engineering services
Discover our molecular biology engineering services for research and innovation: IVT mRNA bioproduction, monoclonal antibody sequencing, DNA and RNA extraction, cell-line validation, amplification method development, and custom engineering.
mRNA biomanufacturing by IVT
Production of research-grade mRNA by in vitro transcription, ideal for research and proof-of-concept applications.
- Sense or antisense transcripts
- Labeled or unlabeled transcripts
- Capped and/or polyadenylated transcripts
- mRNA production at different scales
- Capping
- Incorporation of modified nucleotides
- Polyadenylation through the addition of a poly(A) tail or by post-transcriptional enzymatic processing
- Quantities ranging from a few µg to batches of 5–10 mg
Monoclonal antibody sequencing
Safeguard your hybridomas and rapidly obtain the heavy- and light-chain sequences of your antibodies.
- Backup and secure the sequence of your antibody of interest
- Determine the nucleotide sequences of an antibody’s heavy- and light-chain variable regions for subsequent recombinant antibody production
- Analysis from cultured hybridoma cells, frozen cell pellets, or dry cell pellets, with the corresponding isotype
- Sequencing of the heavy- and light-chain variable regions or full-length antibody sequencing
- Results in under two weeks
- Discounted pricing as part of a complete project: hybridoma generation, vector construction, and recombinant antibody development (murine, human, guinea pig, rat, or other IgG backbones)
Genomic DNA and RNA extraction
We support the preparation, extraction, and purification of RNA and genomic DNA from a wide variety of biological samples, including whole blood, cell pellets, tissues, biopsies, and other biological matrices.
Using optimized, reproducible protocols, we deliver reliable preparations tailored to the requirements of research laboratories, biotechnology companies, and preclinical studies.
- Dedicated RNA and DNA extraction and purification platform
- High-throughput capacity: batches of 50 to 200 samples
- Secure storage of RNA and DNA for subsequent analyses, including RT-PCR, PCR, and cloning
- RNA and DNA quantification by spectrophotometry
- Chip-based RNA integrity assessment
- Agarose gel analysis of RNA and DNA
- Additional quality controls upon request
Cell line validation
This characterization step verifies the correct integration and stability of the transgene in cell lines. It confirms the presence of the transgene at the genomic level and its transcriptional expression through mRNA. This is an essential quality-control step before cell banking or the start of production.
- Verification of transgene presence in the cell line’s genomic DNA by PCR
- Verification of transgene expression through mRNA by RT-PCR
- Verification of transgene sequences
- Preparation of genetic material, including genomic DNA or mRNA extraction and purification
- Development of operating conditions or application of procedures validated by your team
- A clear, detailed analytical report
Development of nucleic acid amplification methods
We address a range of challenges related to gene-expression studies, the analysis of different gene variants, and the identification of specific sequences.
Our services include :
- Optimization or implementation of your validated protocols
- DNA and RNA sample preparation
- Primer design and optimization of amplification conditions
- PCR and RT-PCR amplification
- Sequencing-based quality control of amplicons
- In-depth analysis of results
- Technology transfer
Custom engineering
Need a personalized service? Cannot find exactly what you are looking for in our standard offerings?
Some exemples :
Generation of large dsRNA
Study of splicing variants
Library creation
CRISPR guide design
Development of analytical methods
