Antibody production service
As an expert in monoclonal and recombinant antibody production, RD-Biotech supports your R&D, diagnostic and therapeutic development projects.
Production of monoclonal and recombinant antibodies: benefit from our scientific and technical expertise
Du mg au g
from pilot production to industrial-scale manufacturing
Flexibility
adapted culture media and methods
Clone sécurisé
sequencing and cell banking
Monoclonal antibody sequencing
Secure your clones through high-precision sequencing, converting your hybridomas into genetic sequences ready for engineering and recombinant production.
From mg to grammes
in only 3 weeks
Up to 1 g/L
high-performance production
60+formats
IgG, scFv, VHH, Fab and more
<1 EU/mg
endotoxin-free production
Flexibility
adapted culture media and methods
A panel of labels
HRP, biotin, ALP, FITC and more
Guaranteed quality, made in France
French scientific excellence supporting your innovations through quality, responsiveness, confidentiality and protection of your intellectual property.
RD-Biotech quality applied to antibody production
Accelerate your projects with antibody production combining quality, responsiveness, flexibility and confidentiality. Trusted expertise that turns your scientific challenges into practical solutions.
Why choose RD-Biotech for antibody production?
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Monoclonal antibodies |
Recombinant antibodies |
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Quality: R&D Grade or QualityPLUS
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Benefit from expert, customized support for your antibody development and production projects
« From R&D to therapeutic development, benefit from our scientific and technical expertise in immunology and antibody engineering. Optimize your time while focusing on your major, high-value research priorities »
Quentin Bardin, Recombinant Antibody Production Sales
Meet the team of experts supporting your antibody production projects
Laury
Manager — Hybridoma Antibody Production
Romain
Scientific Project Manager — Recombinant Antibody Production
We meet our customers’ requirements through high-level technical solutions while optimizing their budgets.
Discover our antibody-production expertise through a real life example
Objective: Produce a series of recombinant antibodies from supplied sequences and validate their functional specificity for a tumor-associated membrane target.
- Antibody reformatting: scFv to human IgG1
- Production by transient transfection in HEK293 cells
- Protein A affinity purification, followed by SDS-PAGE and SEC-HPLC analysis
- Characterization using ELISA, flow cytometry and live-cell binding assays
- A comprehensive report supplied in accordance with the customer’s quality requirements
FAQ
Do you have questions about our recombinant antibodies? Here is what you need to know before starting your project.
- Approximately two to three weeks for VH/VL variable-fragment synthesis and subcloning into the expression vector
- Approximately three weeks for production, purification and quality control of the resulting antibody
- RD-Biotech offers a fast-track service enabling pilot-batch production in only four weeks
The customer may supply:
- The target: protein, peptide, protein sequence or bibliographic data
- The murine monoclonal-antibody-secreting clone
- The nucleotide sequences of interest: VH and VL sequences
- Expression vectors ready for transfection
- The cell line
Antibody engineering involves modifying and optimizing antibodies to give them new properties or improve their existing characteristics. For example, antigen-binding sites within the variable regions can be precisely adjusted to increase affinity or specificity for a particular antigen.
- Full-length antibodies from various species, including human, mouse, rat and rabbit
- Antibody fragments such as Fab, scFv and VHH
- Bispecific antibodies
- Chimeric antibodies
- Humanized antibodies
- Fusion proteins
- And more
- Purity testing by SDS-PAGE under reducing and non-reducing conditions
- Capillary electrophoresis using CE-SDS
- Total-protein quantification by absorbance measurement using a spectrophotometer
- Mycoplasma testing
- Determination of bacterial endotoxin levels using an LAL assay
- Testing for bacterial and fungal contamination
- Purity testing by analytical SEC to identify degradation products and aggregates
- Determination of residual Protein A contaminants using the RD-Biotech FastELISA kit
- Quantification of bovine Ig contaminants using the RD-Biotech FastELISA kit
- Molecular-weight verification, identification of post-translational modifications and heterogeneity analysis by mass spectrometry
- Recombinant-antibody specificity and affinity analyses using ELISA, Western blotting, Octet, flow cytometry and customized bioassays
Discover our other services
Since 2002, RD-Biotech has operated as a biotechnology CRO/CDMO, providing customized solutions in molecular biology, immunology and cell engineering for R&D and preclinical and clinical development—with expertise, responsiveness and confidentiality.
Would you like to clarify your expectations?